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We strictly followed iGEM's rules and policies, read over iGEM’s White List and found that we may use organisms not on the White List.
We will extract the cyclic DNA from Escherichia coli DH5α as a carrier to construct tumor-specific nanoparticles. They will be delivered to tumor cells (four tumor cells involving B16-F10, CT26, Panc02 and 4T1 can be involved for efficacy validation), and after invasion through cytotrophy, they will express anti-CD3 scFv (αCD3, a CD3 activator) and anti-CD86 scfv (αCD86, a CD86 activator) on the surface of the tumor cells, and at the same time mobilize the tumor cells to secrete IL- 2 (cytokine for T-cell proliferation and survival) to activate T-cells and enhance the immunotherapy effect.
(1)For human health or safety hazards, they may occur when conducting experiments that require contact with sharp objects, when addressing the needed bacteria or animals like the mouse, or when doing with the chemical materials.
(2)For other hazards to team members or colleagues in the laboratory, they may occur when we are dealing with E. coli or introducing plasmids into tumor cells.
(3)For other hazards beyond laboratory, they may occur when coping with the biowaste.
Our project advisor, and the academic mentor for each of us can support us in managing risks. If we discover a hazard or risk in our project, there are many teachers who can help us, such as our project advisor, the academic mentor for each of us, and other professors and associate professors in the School of Biomedical Science and engineering.
China's laboratory safety laws and regulations cover a wide range of areas, from general guidelines to specific regulations, hazardous chemicals management, legal liability and specific areas of safety management, it provides a comprehensive legal guarantee for the safety management of the laboratory.
In the “Laboratory safety management standard” and “Laboratory Management Regulations”, the detailed provisions of laboratory safety management. The following is a reference to the collation of the detailed provisions on laboratory safety management:
The laboratory must strictly comply with relevant national laws, regulations, and rules to ensure the safety and hygiene of the laboratory and its workplaces. (Article 2: Number 2)
The main responsibilities of safety production management personnel include:
Establish a laboratory safety production manual and safety management system.
Be responsible for the organization, coordination and supervision of safety production work in the laboratory.
Manage the unsafe chemicals in the laboratory and implement safety production measures.
Responsible for safety education and training in the laboratory.
Guide the accident prevention and emergency response work of the laboratory. (Article 2: Number 5)
The laboratory should conduct regular safety education and training, including safety training for new employees, regular safety training for temporary employees, professional training for safety management personnel, and emergency drills and simulations. (Article 2: Number 9)
In order to strengthen laboratory safety management, prevent and reduce laboratory safety accidents, and ensure the orderly conduct of experimental activities and the safety of laboratory personnel and property, (Article 1)
The laboratory safety management work implements a four-level management system of school, institute, experimental center or scientific research team, and laboratory. (Article 1: Number 4)
The school has established a laboratory safety working group, with the school leader in charge of laboratory safety work serving as the group leader. (Article 1: Number 5)
Without permission, no one is allowed to enter the laboratory unless it is for work-related purposes. (Article 4: Number 1)
Undergraduates and graduate students engaged in experiments must follow the operating procedures under the guidance of teachers or experimental technicians. Article 4: Article 2
When using electric heating equipment such as electric stoves and ovens, there should be a designated person to watch over and regularly inspect them, and any problems found should be promptly addressed. (Article 4: Number 4)
The use of highly toxic substances and materials involving biological safety experiments during experiments must be approved by the laboratory head and the designated supervisor of the college. (Article 4: Number 5)
The laboratory should be kept clean and quiet, and safety checks must be conducted before leaving work. (Article 4: Number 6 and 7)
The direct responsible person who violates the safety system, does not comply with the experimental operation procedures, and is irresponsible in work, resulting in accidents, will be held accountable and punished according to the severity of the circumstances. (Article 4: Number 10)
The Regulations on the Safety Administration of Hazardous Chemicals provides detailed provisions on the safety of chemicals. The following are some key legal provisions and regulations in the Regulations, which are summarized and categorized according to different aspects:
Article 3: The definition of "hazardous chemicals" is clarified to refer to highly toxic chemicals and other chemicals that are toxic, corrosive, explosive, flammable, and have the potential to support combustion, which pose a threat to human health, facilities, and the environment.
Article 3: The list of hazardous chemicals is determined and announced by the work safety supervision and management department of the State Council in conjunction with other relevant departments, and is adjusted in a timely manner.
Article 4: It is emphasized that the safety management of hazardous chemicals should adhere to the principle of safety first, prevention first, and comprehensive management, and strengthen and implement the main responsibility of enterprises.
Article 6: It stipulates in detail the safety supervision and management responsibilities of relevant departments in the production, storage, use, operation, and transportation of hazardous chemicals.
The department of work safety supervision and management is responsible for comprehensive work, including determining, publishing, adjusting the list of hazardous chemicals, and issuing relevant licenses.
Public security organs: responsible for public safety management, issuing permits for the purchase of highly toxic chemicals, road transportation passes, etc.
Quality supervision, inspection and quarantine department: responsible for issuing relevant certificates for hazardous chemicals and their packaging materials and containers.
Article 4: It is stipulated that units that produce, store, use, operate, and transport hazardous chemicals shall provide safety education, legal education, and job-specific technical training for their employees to ensure that they have the appropriate safety knowledge and skills.
Article 5: It is clearly stipulated that no unit or individual may produce, operate, or use hazardous chemicals that are prohibited by the state from being produced, operated, or used. At the same time, for hazardous chemicals with restrictive regulations, no unit or individual may use them in violation of the restrictive regulations.
The regulations also stipulate the legal responsibilities that should be borne by units and individuals who violate the above provisions, including administrative penalties and criminal responsibilities.
Article 125 of the Criminal Law stipulates corresponding criminal responsibility for those who illegally manufacture, trade, transport, and store toxic, radioactive, infectious disease pathogens and other substances that endanger public safety. Article 30 of the Law on Public Security Administration Punishments stipulates that corresponding administrative penalties shall be imposed on those who violate state regulations by buying, selling, storing, transporting, mailing, using, providing, or disposing of explosive, toxic, radioactive, corrosive substances or infectious disease pathogens and other dangerous substances.
Regarding the use and safety of the laboratory, the school has developed a detailed safety code, which is strictly followed by our project team members, not only can help us to avoid most of the security problems caused by improper operation, but also can help us standardize the experimental operation.
Before we entered the laboratory, the school carried out collective training on laboratory safety. Only those who had participated in the whole course and achieved full marks in the laboratory safety knowledge test could enter the laboratory, which allows each student into the laboratory have enough knowledge of safety, with the ability to solve most of the safety problems, to further ensure the safety of the laboratory.
Under the leadership of our seniors and mentors, we have the right to use cell laboratories and animal laboratories. Under the leadership of our experienced seniors, we can effectively circumvent laboratory safety risks and standardize safe operations, at the same time flexible response to possible security issues.
The laboratory will collect the waste from the experiment and hand it over to the specialist for disposal.