Section 1:Introduction
The modularization and standardization of genetic components in the field of synthetic biology is an important area of current research.
Modularization allows genetic elements to be combined like LEGO blocks, simplifying the design and construction of complex biological systems. This approach enables researchers to reuse the same components across different projects, increasing efficiency and reproducibility.
Standardization provides a foundation for collaboration between different laboratories and researchers, ensuring that the genetic components used are consistent. This not only facilitates the sharing of data and resources but also promotes the development of biological component databases, such as BioBrick and the iGEM repository, fostering communication and the spread of technology.
In this year's project, our team registered 8 basic parts and 5 composite parts, all in compliance with the requirement of RFC[10] or RFC[1000]. During the combination of basic parts to create composite parts, we focused on the role of each basic part within the overall construct. Through iterative sequence design and optimization, we aim to ensure that everyone can access our parts information on the Registry for further research and applications.
Section 2: Basic parts
Part Numbers | Name | Type | Length |
---|---|---|---|
BBa_K5499000 | CMV enhancer | Regulatory | 304 |
BBa_K5499001 | CMV promoter | Regulatory | 204 |
BBa_K5499002 | IDS | Coding | 1650 |
BBa_K5499003 | Exosignal | Coding | 57 |
BBa_K5499005 | LAMP2A signal peptide | Coding | 84 |
BBa_K5499006 | Trans-activator of transcription (TAT) |
Coding | 33 |
BBa_K5499007 | LAMP2A mature peptide | Coding | 1146 |
BBa_K5499008 | P2A | Intermediate | 66 |
BBa_K5499009 | Flexible linker | Intermediate | 30 |
Section 3: Composite parts
Part Numbers | Name | Type | Length |
---|---|---|---|
BBa_K5499004 | CMV enhancer-CMV promoter | Composite | 508 |
BBa_K5499013 | Engineered Exosome 1 | Composite | 2158 |
BBa_K5499010 | Engineered Exosome 2 | Composite | 1911 |
BBa_K5499011 | Engineered Exosome 3 | Composite | 1869 |
BBa_K5499012 | Engineered Exosome 4 | Composite | 3604 |