SYSTEM 1
SYSTEM 2
SYSTEM 3
SYSTEM 4

SYSTEM 1 -Tetrahydrofolate for the treatment of Alzheimer's disease

Basic Parts

Number	Name	Type	Length	Description
BBa_K5238001	folKE	Coding	1075bp	Production of tetrahydrofolate as our target product to bind to target lilrb3 for the treatment of Alzheimer's disease
BBa_K387003	PfdhF	Promoter	99bp	Hypoxia-inducible promoter. Under hypoxia environment it has a much higher efficiency of starting transcription than under normal 20% oxygen pressure.
BBa_B0034	RBS	RBS	12bp	RBS based on Elowitz repressilator
BBa_K1725060	SrpR repressor	Coding	642bp	encodes an SrpR repressor that inhibits transcription of the SrpR-dependent promoter (BBa_K1725020).
BBa_K2978212	Terminator	Terminator	73bp	Terminator for Lactobacillus spp
BBa_K1725020	SrpR repressible promoter	Promoter	67bp	SrpR repressible promoter which can be repressed by SrpR repressor(BBa_K1725060)
BBa_B0034	RBS	RBS	12bp	RBS based on Elowitz repressilator.
BBa_K4133012	LysKB317	Coding	894bp	LysKB317 is a novel recombinant peptidoglycan hydrolase (endolysin) derived from Lactobacillus bacteriophage vB_LfeS_EcoSau, with the ability of inhibiting the growth of lactic acid bacteria
BBa_K2978212	Terminator	Terminator	73bp	Terminator for Lactobacillus spp

Composite Parts

Number	Name	Type	Length	Description
BBa_K5238005	Promoter-folKE	Composite	1194bp	For this composite part we want to produce of tetrahydrofolate as our target product to bind to target lilrb3 for the treatment of Alzheimer's disease
BBa_K5238006	PfdhF-SrpR repressor	Composite	826bp	This composite part include a Hypoxia-inducible promoter,a RBS,a coding of SrpR repressor that inhibits transcription of the SrpR-dependent promoter and a terminator. Under low oxygen conditions, it can induce the production of downstream inhibitory proteins, thereby inhibiting endotoxins and promoting the survival of Lactobacillus plantarum under low oxygen conditions
BBa_K5238008	SrpR repressible promoter-LysKB317	Composite	973bp	This composite part include a SrpR repressible promoter,a RBS,a coding of LysKB317 (endolysin)and a terminator. To achieve suicide of Lactobacillus plantarum under non hypoxic conditions
BBa_K5238009	PfdhF-SrpR repressor-SrpR repressible promoter-LysKB317	Composite	1799bp	The entire section is a suicide switch. Under conditions of intestinal hypoxia, the production of suicide proteins is inhibited, while extracellular factors can cause the expression of endoplasmic reticulum, leading to suicide

SYSTEM 2 -inosine for the treatment of depression

Number	Name	Type	Length	Description
BBa_K5238003	gsk	Coding	1305bp	For the treatment of depression we want produce inosine to

SYSTEM 3 -theanine for the treatment of both Alzheimer's disease & depression

Number	Name	Type	Length	Description
BBa_K5238002	glnA	Coding	1344bp	For this system we anticipate to produce theanine to treat Alzheimer's disease & depression

SYSTEM 4 -niacin for the treatment of both Alzheimer's disease & depression

Number	Name	Type	Length	Description
BBa_K5238004	LBP_cg2111	Coding	621bp	For this system we anticipate to produce theanine to treat Alzheimer's disease & depression

Design safety-part

Name	Number	Type	Length	Description
PsppA	BBa_K5238012	Other	137bp	The PsppA promoter is a strong inducible promoter that regulates gene expression in Lactobacillus plantarum.
PsppA+LysKB317	BBa_K5238013	Composite	1031bp	The PsppA promoter controls the expression of the endosome LysKB317, leading to the programmed cell death of Lactobacillus plantarum.
PfdhF-SrpR-PsrpR-LySKB317	BBa_K5238009	Composite	1799bp	The entire system acts as a suicide switch; under intestinal hypoxic conditions, the synthesis of suicide proteins is suppressed, but when exposed to high concentrations of oxygen, the expression of suicide proteins is induced, facilitating the self-destruction of Lactobacillus plantarum through apoptosis.
P9-folKE-PfdhF-SrpR-PsrpR-LySKB317	BBa_K5238015	Composite	3149bp	Expression System and Induced Suicide Switch.

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njmuigem2021@163.com

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