Overview Parts
Parts
Overview

Our components are designed in a modular fashion, which not only facilitates use by our team but also by other teams with similar objectives. To address food waste, specifically coffee grounds, we employed synthetic biology to convert the caffeine they contain into a high-value compound, 7-MX, which has potential applications in treating myopia. To achieve this, we utilized several components from the Ndm enzyme family. In addition, we drew on the designs and experiences of previous iGEM teams, simplifying our experimental process and improving our overall results. Modular design is a core principle of synthetic biology, allowing production systems to produce entirely different outcomes with only minor modifications. This approach enables us not only to achieve notable success in this project but also to quickly apply our methods to the production of other homologous compounds by swapping modules. This framework will be especially beneficial to future iGEM teams by providing both design insights and key components. It aligns with iGEM's ethos of fairness, collaboration, and mutual learning. We hope that our PARTS will benefit many teams, further demonstrating the meaningful impact of our work.

Type Features Part URL Name Description
Basic coding BBa_K5313012 ndmA Ndm/D have specific N-1 demethylase activity
Basic coding BBa_K5313013 ndmDt NdmD without its first 266 N-terminal amino acids
Basic coding BBa K5313015 frmA Interacting with FrmB and FDH to oxidize 1 molecule formaldehyde and produce 2-molecule NADH
Basic coding BBa_K5313016 frmB Interacting with FrmA and FDH to oxidize 1 molecule formaldehyde and produce 2-molecule NADH
Basic coding BBa_K5313017 FDH Interacting with FrmB and FrmA to oxidize 1 molecule formaldehyde and produce 2-molecule NADH
Basic coding BBa_K5313018 ndm B NdmB/D have specific N-3 demethylase activity
Basic coding BBa_K5313019 ndm BQ289T Mutants of ndmB
Basic coding BBa_K5313020 ndm BQ289S Mutants of ndmB
Basic coding BBa_K5313021 ndm BQ289A Mutants of ndmB
Basic coding BBa_K5313022 ndm C ndmC/D/E have specific N-7 demethylase activity
Basic coding BBa_K5313023 ndm E ndmC/D/E have specific N-7 demethylase activity
Basic coding BBa_K5313024 guaB Typically encodes GMP synthetase, an enzyme involved in the purine biosynthesis pathway, critical for the production of guanosine monophosphate (GMP).
Composite plasmid BBa_K5313025 CRS Cofactor recycling system
Composite plasmid BBa_K5313026 pYB1s-ndmDtBs Template for constructing an ndmB mutant library
Composite plasmid BBa_K5313027 pYB1s-ndmDtA Ndm/D have specific N-1 demethylase activity
Composite plasmid BBa_K5313028 pYB1s-ndmDBCAE Removal of methyl groups at positions 1, 3, and 7 of caffeine
Composite plasmid BBa_K5313029 pYB1s-ndmDCE ndmC/D/E have specific N-7 demethylase activity
Composite plasmid BBa_K5313030 pYB1s-NdmDtBA Used for the synthesis of 7-MX to test the biosensor
Composite plasmid BBa_K5313034 pKD46 Used for gene knockout via Red homologous recombination
NORTHEAST FORESTRY UNIVERSITY
NEFU-China
Welcome to subscribe us
iGEM_Work@163.com
©NEFU-China Licensed under Creative Commons Attribution 4.0 Source code available here