Our components are designed in a modular fashion, which not only facilitates use by our team but also by other teams with similar objectives. To address food waste, specifically coffee grounds, we employed synthetic biology to convert the caffeine they contain into a high-value compound, 7-MX, which has potential applications in treating myopia. To achieve this, we utilized several components from the Ndm enzyme family. In addition, we drew on the designs and experiences of previous iGEM teams, simplifying our experimental process and improving our overall results. Modular design is a core principle of synthetic biology, allowing production systems to produce entirely different outcomes with only minor modifications. This approach enables us not only to achieve notable success in this project but also to quickly apply our methods to the production of other homologous compounds by swapping modules. This framework will be especially beneficial to future iGEM teams by providing both design insights and key components. It aligns with iGEM's ethos of fairness, collaboration, and mutual learning. We hope that our PARTS will benefit many teams, further demonstrating the meaningful impact of our work.
Type | Features | Part URL | Name | Description |
---|---|---|---|---|
Basic | coding | BBa_K5313012 | ndmA | Ndm/D have specific N-1 demethylase activity |
Basic | coding | BBa_K5313013 | ndmDt | NdmD without its first 266 N-terminal amino acids |
Basic | coding | BBa K5313015 | frmA | Interacting with FrmB and FDH to oxidize 1 molecule formaldehyde and produce 2-molecule NADH |
Basic | coding | BBa_K5313016 | frmB | Interacting with FrmA and FDH to oxidize 1 molecule formaldehyde and produce 2-molecule NADH |
Basic | coding | BBa_K5313017 | FDH | Interacting with FrmB and FrmA to oxidize 1 molecule formaldehyde and produce 2-molecule NADH |
Basic | coding | BBa_K5313018 | ndm B | NdmB/D have specific N-3 demethylase activity |
Basic | coding | BBa_K5313019 | ndm BQ289T | Mutants of ndmB |
Basic | coding | BBa_K5313020 | ndm BQ289S | Mutants of ndmB |
Basic | coding | BBa_K5313021 | ndm BQ289A | Mutants of ndmB |
Basic | coding | BBa_K5313022 | ndm C | ndmC/D/E have specific N-7 demethylase activity |
Basic | coding | BBa_K5313023 | ndm E | ndmC/D/E have specific N-7 demethylase activity |
Basic | coding | BBa_K5313024 | guaB | Typically encodes GMP synthetase, an enzyme involved in the purine biosynthesis pathway, critical for the production of guanosine monophosphate (GMP). |
Composite | plasmid | BBa_K5313025 | CRS | Cofactor recycling system |
Composite | plasmid | BBa_K5313026 | pYB1s-ndmDtBs | Template for constructing an ndmB mutant library |
Composite | plasmid | BBa_K5313027 | pYB1s-ndmDtA | Ndm/D have specific N-1 demethylase activity |
Composite | plasmid | BBa_K5313028 | pYB1s-ndmDBCAE | Removal of methyl groups at positions 1, 3, and 7 of caffeine |
Composite | plasmid | BBa_K5313029 | pYB1s-ndmDCE | ndmC/D/E have specific N-7 demethylase activity |
Composite | plasmid | BBa_K5313030 | pYB1s-NdmDtBA | Used for the synthesis of 7-MX to test the biosensor |
Composite | plasmid | BBa_K5313034 | pKD46 | Used for gene knockout via Red homologous recombination |