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Contribution

Based on the human-plant-microbe symbiotic system developed by our team, we have provided a brand-new solution for space life support systems, offering astronauts a detailed operation manual, including how to cultivate microbes, generate electricity, and manage circuits. We described the different stages of microbial growth and the required environmental conditions, helping astronauts to monitor and adjust the growth status of microbes, ensuring the reliability and user-friendliness of the system in the space environment.


1. New Documentation to Existing Parts

We've added new documentation to the BBa_B0034 where we submit the results of how we utilize the element, how it's built in our plasmid, and how it's tested. We have proven the following:

  1. The element can be expressed in S.oneidensis.
  2. When the element is expressed in S.oneidensis, it results in a relatively high amount of protein expression downstream. We also performed a polyacrylamide gel electropherogram to demonstrate this.

In addition to this, we also provide annotations for BBa_K3022002. We demonstrated the following:

  1. This element can be expressed in S.oneidensis after codon optimization. We provide polyphosphorus data for PPK1 in S.oneidensis.
  2. The role of this element in S.oneidensis converts Pi to PolyP, and also affects the power production process of S.oneidensis. We provide the power production data of S.oneidensis.
  3. The high expression level of this element in S.oneidensis may affect the morphology of S.oneidensis, resulting in significant changes in the morphology of S.oneidensis. We provide images of transmission electron microscopy and scanning electron microscopy.
  4. The large expression of this element in S.oneidensis will lead to the death of S.oneidensis. This is reflected in the growth curve. We also provide data on growth curves.

2. EGOAL: a gene Expression prediction model based on Gene Ontology and Abductive Learning

EGOAL is an attempt of introducing the Holy Grail of Artificial Intelligence — combination of Machine Learning and Symbolic Reasoning, aka. Neural-Symbolic AI — into the sight of Synthetic Biology.

We are trying to assist researches and drive innovations in SynBio, via Abductive Learning, the novel paradigm of neural-symbolic AI proposed by researchers from Nanjing University.

Our project code is available at github.com/yfxiang0112/EGOAL. Everyone can predict their experiments with our project freely.


3. User Manual for Astronaut

Based on the human-plant-microbe symbiotic system developed by our team, we have provided a brand-new solution for space life support systems, offering astronauts a detailed operation manual, including how to cultivate microbes, generate electricity, and manage circuits. We described the different stages of microbial growth and the required environmental conditions, helping astronauts to monitor and adjust the growth status of microbes, ensuring the reliability and user-friendliness of the system in the space environment.

See “Support” page.


4. Visual Standards Guideline

The Nanjing-China team has established a visual identity process for the iGEM competition, aiming to ensure that our project "Shivacosmic Greens" presents a consistent and professional image in various displays and print materials. Nanjing-China summarized relevant insights and, within the framework of Chinese culture, introduced a Visual Standards Checklist to assist teams in step-by-step development of their own visual standards.

See also “Support” page.


5. Documentation of Our Activities

In our various human practice activity records, we have set up a "learning" section, which can provide experience and new inspiration and strategies for solving practical problems for our team, other iGEM teams, and a broader range of stakeholders.

See “Activities” page.


6. Legal Advice Sharing

Our visit to the SINO PRO LAW FIRM in Beijing has provided us with legal advice on current carbon emission trading issues in China, allowing us to provide more detailed successful cases and policy guidance for reducing environmental pollution.

In view of the complexity of the China Carbon Emissions Trading Exchange (CCETE), we have decided to publicly share relevant legal guidance to assist more iGEM teams working on SDG-related projects, providing a reference for all teams. See “SDGs” page.


7. Picture Book

On April 13, 2024, Nanjing-China had an online meeting with the Fudan iGEM team, SJTU-bioX-Shanghai, NUDT-CHINA, ZJU-China team. We have preliminarily discussed and determined to collaborate on creating a synthetic biology-themed picture book design to promote the popularization of synthetic biology in education and further expand its influence.

Even though the script wasn't finalized, we put together a collection of material for other people to use. See “Support” page.


8. Hardware

For the convenience of other teams in the iGEM community working on microbial fuel cells, we have chosen to make the circuit design document public. Any iGEM team can refer to our design.See “Hardware” page.