Click each month to expand and display individual notebook pdf files linked by week
May
June
July
May
August
September
May
June
Week 1: 6/3 - 6/7
Week 3: 6/17 - 6/21
- Cultured stocks
- Made LB agar Gent. Plates, Gel Extract Digested Plasmid, Nanodrop Digested Plasmid
- Re-ran Gel Extract, Ran Gibson Assembly using Heat Shock, Streak Gent. Plates
- Cultured Colonies
- Miniprep NicA2 E.coli, Restriction Enzyme Digested the Miniprep, Ran the Gel, and Heat Shocked the plasmid into the competent cells
Week 4: 6/24 - 6/28
- Ran Gel on Gibsoned Samples
- Colony PCR to confirm THFMO Transformed in E.coli, Made Glycerol Stocks
- Made P.putida Overnights, Made a Plate of Culture 3 in E.coli, Miniprepped and Made a Stock of Culture 3, Ran Gel on Colony PCR Samples
- Miniprepped Overnight Samples, Ran Gel on Miniprep, Nanodropped miniprep
July
Week 1: 7/1 - 7/5
Week 3: 7/15 - 7/20
- Check on P. putida plates and start new overnights of P. putida and E. coli
- Make glycerol stocks, Grow up cultures and induce for overexpression
- Set up degradation assay of dioxane
- Take degradation assay samples at time points of 12 hours and 20 hours, and make arabinose solution
- Take degradation assay endpoint samples, and spin down to collect cells
August
Week 1: 7/29-8/2
Week 3: 8/12 - 8/16
- Started R2A Overnights, Checked Triplicate Cultures
- Diluted Cultures, Checked OD of Cultures
- Tested Degradation of Overnight Cultures (grown in LB) in Triplicate, Collected Samples Hourly for 12 Hours
- Tested Degradation of Overnight Cultures (grown in R2A) in Singlet, Collected Samples Hourly for 12 Hours, then every 4 Hours until 36 Hours