1. New Basic Parts

We registered and uploaded basic parts BBa_K5392000, BBa_K5392001, BBa_K5392002, BBa_K5392003, BBa_K5392004, BBa_K5392006 and BBa_K5392007

2. New Composite Parts

We have designed eight composite parts (BBa_K5392015, BBa_K5392016, BBa_K5392017, BBa_K5392018, BBa_K5392019, BBa_K5392020, BBa_K5392021 and BBa_K5392022) in order to overexpress the corresponding genes in cells.

3. All Parts We Made

Part Number	Part Name	Part type	Abstract
BBa_K5392000	ZaTdT	Coding	ZaTdT is a Terminal deoxynucleotidyl transferase gene from Zonotrichia albicollisnhad.
BBa_K5392001	ZaTdT-R335P	Coding	ZaTdT-R335P is a mutant type of ZaTdT
BBa_K5392002	ZaTdT-R335W	Coding	ZaTdT-R335W is a mutant type of ZaTdT
BBa_K5392003	ZaTdT-R335I	Coding	ZaTdT-R335I is a mutant type of ZaTdT
BBa_K5392004	ZaTdT-R335F	Coding	ZaTdT-R335F is a mutant type of ZaTdT
BBa_K5392005	ZaTdT-R335M	Coding	ZaTdT-R335M is a mutant type of ZaTdT
BBa_K5392006	ZaTdT-K337A	Coding	ZaTdT-K337A is a mutant type of ZaTdT
BBa_K5392007	ZaTdT-K337L	Coding	ZaTdT-K337L is a mutant type of ZaTdT
BBa_K5392015	pET28a-ZaTdT	Plasmid	We loaded the genes encoding ZaTdT into plasmid vectors. In this way we were able to over-express the in the target cells.
BBa_K5392016	pET28a-ZaTdT-R335P	Plasmid	We loaded the genes encoding ZaTdT-R335P into plasmid vectors. In this way we were able to over-express the in the target cells.
BBa_K5392017	pET28a-ZaTdT-R335W	Plasmid	We loaded the genes encoding ZaTdT-R335W into plasmid vectors. In this way we were able to over-express the in the target cells.
BBa_K5392018	pET28a-ZaTdT-R335I	Plasmid	We loaded the genes encoding ZaTdT-R335I into plasmid vectors. In this way we were able to over-express the in the target cells.
BBa_K5392019	pET28a-ZaTdT-R335F	Plasmid	We loaded the genes encoding ZaTdT-R335F into plasmid vectors. In this way we were able to over-express the in the target cells.
BBa_K5392020	pET28a-ZaTdT-R335M	Plasmid	We loaded the genes encoding ZaTdT-R335M into plasmid vectors. In this way we were able to over-express the in the target cells.
BBa_K5392021	pET28a-ZaTdT-K337A	Plasmid	We loaded the genes encoding ZaTdT-K337A into plasmid vectors. In this way we were able to over-express the in the target cells.
BBa_K5392022	pET28a-ZaTdT-K337L	Plasmid	We loaded the genes encoding ZaTdT-K337L into plasmid vectors. In this way we were able to over-express the in the target cells.

Ultimately, we found that BBa_K5392000 was new basic part because it had better catalytic activity for natural dNTP than other sources of TdTs and BBa_K5392021 was new composite part, because they obtained better catalytic activity compared to wild-type ZaTdT or other ZaTdT mutant for 3’-O-(2-nitrobenzyl)-dNTP that is a 3’-O-blocked reversible terminator.