Introduction to Bioimaging:
Bioimaging is a crucial technology for advancing our understanding of biological systems. It is widely used in medical diagnostics, surgical guidance, and research to visualize structures within living organisms. However, current imaging technologies face significant limitations in terms of depth penetration, resolution, and background interference.
Comparison of Penetration Depth between Lower-wavelength Fluorescence and NIR Fluorescence
Current Challenges:
Despite the importance of bioimaging, traditional methods using visible or near-infrared I (NIR-I) probes are limited by poor tissue penetration and high autofluorescence, which reduce image clarity. These issues become particularly prominent when imaging deep tissues or delicate biological structures, where greater precision and longer imaging times are required. Additionally, most probes suffer from poor stability in vivo, leading to short circulation times and suboptimal imaging quality.
Our Solutions
Our project addresses these challenges by developing a near-infrared II (NIR-II) biomimetic fluorescent protein probe. By genetically engineering human serum albumin (HSA), we significantly enhance the binding efficiency and stability of the NIR-II probe, greatly improving its performance in deep-tissue imaging. This biomimetic design not only reduces background interference but also extends the probe’s circulation time, allowing for more precise and clearer imaging results. This innovative system has broad applications in various fields of biological research and clinical diagnostics, providing a powerful tool for next-generation imaging needs.
