While sifting through different protocols and newer systems of SELEX, we chanced upon the work of an iGEM team, OLM Madrid, that had tried to develop a more efficient system of SELEX. We consulted Laura Armero Hernandez from their team.
The meeting (facilitated via an online platform) provided many insights about SELEX, how tedious it can be, and the effectiveness of the result it provides. The method they used also took around 3-4 months to produce an effective and more selective aptamer. Considering the time constraint and our plan of action, Laura suggested that we avoid performing SELEX (since it was not the central feature of our idea but only a part of it). She instead encouraged us to use pseudo-SELEX or directly use an established aptamer.
