Our Contributions to the iGEM Community

The real aim of the project was to set a precedent in the approach of targeting and clearing misfolded proteins using Aptamers. We used Tau as a model and phosphorylation as the label. We have a few registry parts which may be useful to future teams working with Tau or implementing any therapeutics against Tauopathies.

Registry Parts	Designers	Short Description	Type
BBa_K5170000	Subhanan Banerjee, Suvam Saswat Das, Avani, Anurag Sarkar, Parth Kumar	A Novel PROTAC using BBa_K5170004	Conjugate
BBa_K5170001	Subhanan Banerjee, Suvam Saswat Das, Avani, Anurag Sarkar, Parth Kumar	A Novel PROTAC using BBa_K5170002	Conjugate
BBa_K5170002	I-Ting Teng, Xiaowei Li, Hamad Ahmad Yadikar, Zhihui Yang, Long Li, Yifan Lyu, Xiaoshu Pan, Kevin K. Wang, Weihong Tan	DNA Aptamer against 231pTau	DNA
BBa_K5170003	I-Ting Teng, Xiaowei Li, Hamad Ahmad Yadikar, Zhihui Yang, Long Li, Yifan Lyu, Xiaoshu Pan, Kevin K. Wang, Weihong Tan	DNA Aptamer against 231pTau	DNA
BBa_K5170004	I-Ting Teng, Xiaowei Li, Hamad Ahmad Yadikar, Zhihui Yang, Long Li, Yifan Lyu, Xiaoshu Pan, Kevin K. Wang, Weihong Tan	DNA Aptamer against 231pTau	DNA
BBa_K5170005	Subhanan Banerjee, Suvam Saswat Das, Avani, Anurag Sarkar, Parth Kumar	A Novel PROTAC using BBa_K5170003	Conjugate
BBa_K5170006	Suvam Saswat Das	Phosphomimetic Tau compatible with pET29b vector	Protein Domain

alt — The modified ligand linked base can joined in the 3' terminal of an Aptamer. This is a tool that can convert the activity of the aptamer into that of a PROTAC upon incorporation by Terminal Deoxynucleotidyl Transferase.

alt — Illustration of the modified ligand linked base bound with the 3’ of the Aptamer (A-T-G-T-modified C)

alt — BBa_K5170006 shown in pET29b vector, highlighted in blue. It can be expressed in E. coli BL21 via this vehicle.