Our project aims to boost bacterial cellulose (BC) production in Komagataeibacter xylinus and utilize it as a water-retaining substrate to enhance seedling survival rates in desert environments, addressing the issue of desertification. In the process of achieving our project goals, we designed and constructed the following components. Among these components, we thoroughly characterized the composite part BBa_K5507008. We constructed BBa_K5507008 based on BBa_K5507009 through Gibson assembly and successfully obtained the pgi gene overexpressing strain of Komagataeibacter xylinus via electroporation. Growth curve measurements indicated that this part does not affect bacterial growth and metabolism but significantly increases bacterial cellulose production by approximately 111%. Additionally, we evaluated the water retention capacity of the BC product in aiding soil. The results showed that the water loss rate of the BC group was noticeably slower, about 50% less than that of the control group. Therefore, BBa_K5507008 can increase the bacterial cellulose yield in Komagataeibacter xylinus, and the application of BC during tree planting helps to slow soil moisture loss, thereby improving seedling survival rates. Furthermore, the increased BC yield is beneficial for reducing the costs of using this material in afforestation.