Introduction
The field of biology is distinct from other engineering disciplines due to the absence of a modular
structure and function. This results in alterations to the expression of genes when they are transferred
between species. The objective of synthetic biology is to standardise functional genes in order to
facilitate predictable expression in different chassis organisms. Our project has registered 17 basic
parts and 3 composite parts. It is our hope that these parts will be accessible for utilisation based
on our research through the registry, and that the information for the parts can be further completed
so that more people can make use of them.
Among these parts, we choose turboPETase(OmpA), (BBa_K5233022) as our best new basic part for the application of gold medal.
Basic Parts
| Code | Name | Description |
|---|---|---|
| BBa_K5233025 | TurboPETase(LamB) | A designed enzyme that can be secreted into the extracellular space |
| BBa_K5233021 | TurboPETase(MalE) | A designed enzyme that can be secreted into the extracellular space |
| BBa_K5233022 | TurboPETase(OmpA) | A designed enzyme that can be secreted into the extracellular space |
| BBa_K5233023 | TurboPETase(PelB) | A designed enzyme that can be secreted into the extracellular space |
| BBa_K5233006 | tpiA | A kind of tripartite tricarboxylate transporter(TTT) family protein |
| BBa_K5233007 | tpiB | A kind of tripartite tricarboxylate transporter(TTT) family protein |
| BBa_K5233008 | tphC | tphC, a kind of substrate-binding protein (SBP) belonging to the tripartite tricarboxylate transporters |
| BBa_K5233009 | tphR | A kind of regulatory protein that can form complex with TPA which will activate PtphC |
| BBa_K5233010 | PtphC | PtphC is a kind of promter that responds to the presence of TPA |
| BBa_K5233011 | eGFP | A kind of green fluorescent protein |
| BBa_K5233012 | MHETase(Amcut) | A designed enzyme that can be secreted into the extracellular space and degrade MHET |
| BBa_K5233013 | MHETase(pelB) | A designed enzyme that can be secreted into the extracellular space and degrade MHET |
| BBa_K5233000 | vha-6 | A specific promoter that only expressed in the gut of C.elegans |
| BBa_K5233040 | gbb-1 | A kind of signal peptide that can guide protein locate on Intestinal cell membrane, it is used for protein that has 7 transmembrane domains |
| BBa_K5233050 | glr-2 | A kind of signal peptide that can guide protein locate on Intestinal cell membrane, it is used for protein that has 3 transmembrane domains |
| BBa_K5233042 | dsl-1 | A kind of signal peptide that can guide protein secretion |
| BBa_K5233043 | SL2 | Spliced leader 2 |
Composite parts
| Code | Name | Description |
|---|---|---|
| BBa_K5233044 | tpiA-tpiB-tphC | A transporter element, an element that can transport TPA into E. coli |
| BBa_K5233030 | tphR-PtphC-eGFP | A biosensor element, an element that can detect the degradation of PET |
| BBa_K5233032 | vha6-gbb1-tpiA-SL2-glr2-tpiB-SL2-dsl1-tphC | A transporter system in C.elegans, a system that can transport TPA into the C.elegans intestinal cell |