| BBa_K5116006
| FIG1-GalE
| composite
| When the fungal pheromone receptor responds, the signaling pathway is activated, which enables ste12 to enter the nucleus and bind to promoter FIG1 to promote GalE expression.
|
| We chose Sc-pCYC1 that is a regular promoter to stabilize the expression of B4GalT1.
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| BBa_K5116009
| Sc-pCYC1-lactalbumin α
| composite
| We chose Sc-pCYC1 that is a constitutive promoter to stabilize the expression of lactalbumin α.
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| BBa_K5116010
| PpltR-LacO
| composite
| We could not find the regulatory promoter of Pseudomonas fluorescence, but we took inspiration from the lactose regulatory system and used lacO and PpltR to construct a artificial promoter that can transmit information between the two systems of Pseudomonas and yeast。
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| BBa_K5116004
| pseudomonas secretion signal peptide
| Basic
| A signaling peptide of Pseudomonas fluorescens that enables it to secrete proteins outside the capsule.
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| BBa_K5116011
| pseudomonas secretion signal peptide-Brevenin1-Ma
| composite
| This is our core component, Brevenin 1-MA is an antimicrobial peptide that kills the Batrachochytrium dendrobatidis, and this signaling peptide directs its secretion to the outside of the capsule.
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| BBa_K5116012
| PpltR-LacO-pseudomonas secretion signal peptide-Brevenin1-Ma
| composite
| The complete peptide expression system, which is expressed when the lactose signal is emitted upstream
|
| BBa_K5116013
| PampC-LacY
| composite
| Gives the ability of Pseudomonas fluorescens to absorb lactose.
|
| BBa_K5116015
| pET28a(+)-PampC-LacY-PpltR-LacO-pseudomonas secretion signal peptide-Brevenin1-Ma
| composite
| This is the core plasmid that we transfer into Pseudomonas, through which we absorb lactose, receive lactose signaling, promote the expression of antimicrobial peptides through lactose operons, and enable them to be secreted outside the cell to kill Batrachochytrium dendrobatidis
|
| BBa_K5116018
| pGADT7-CYC1-B4GalT1
| composite
| A plasmid that expresses B4GalT1，which codes a enzyme that can transfer a glycosidic acid(usually glucose uridine diphosphate or galactose urdine diphosphate) to a molecule.
|
| BBa_K5116019
| pGBKT7-CYC1-LALBA-FIG1-GalE
| composite
| A plasmid that expresses UDP-glucose epimerase and lactalbumin α which forms the lactose synthesis complex with UDP- galactosyltransferase in pGADT7
|
