l o a d i n g
Parts

Overview

HBUT-Wuhan team added 11 new parts to the iGEM registry, including 8 basical parts and 3 composite parts. In order to produce small molecular weight polypeptide Vg in Pichia pastoris, we selected a methanol-induced vector pPIC9K, which contains AOX promoter, secretion signal peptide, resistance gene and so on. In addition, in order to increase the expression yield of polypeptides or facilitate the purification of products, we introduced fusion proteins, His-tag, acid-sensitive sites and other components. All the new elements have been submitted to the iGEM parts registry to comply with the bio-brick assembly standard. Herein, we briefly described the Parts section in the following table. The detailed information of each part can be reached by clicking on the term ‘part name’ in the table.

Basical Part

Part number Name Description Type
BBa_K5384001
parts.igem.org
Vglycin A hypoglycemic peptide Coding
BBa_K5384002
parts.igem.org
DDDDK A sequence that can be specifically recognized and cleaved by an enterokinase. Scar
BBa_K5384003
parts.igem.org
Asp-Pro A acid-sensitive sites between the fusion parter and antimicrobial, using acid to cleave fusion peptide. Scar
BBa_K5384004
parts.igem.org
AOX 1 promoter In our experiment, it serves as a strong promoter for the expression of most proteins. Signalling
BBa_K5384005
parts.igem.org
α-secretion signal peptide A signal peptide can guide recombinant protein be secreted to extra cellular space. Signalling
BBa_K5384006
parts.igem.org
His-tag As a tag for our recombinant detection. Signalling
BBa_K5384010
parts.igem.org
Nattokinase An alkaline serine endopeptidase secreted by Bacillus subtilis(natto) Coding
BBa_K5384011
parts.igem.org
AOX 1 teminater The most commonly used terminator Terminator

Composite Part

Part number Name Description Type
BBa_K5384007
parts.igem.org
pPIC9K-His-DDDDK-Vg Using DDDDK as restriction site, pPIC9K as vector to express Vglycin. Plasmid
BBa_K5384008
parts.igem.org
pPIC9K-3Vg-His Using Asp-Pro as isolation site, pPIC9K as vector to express Vglycin. Plasmid
BBa_K5384009
parts.igem.org
pPIC9K-NK-3Vg-His Using Asp-Pro as isolation site, pPIC9K as vector to express NK and Vglycin. Plasmid