Parts
On this page, you will find a detailed description of the biological parts we’ve developed, each designed with specific applications in synthetic biology. These parts range from simple genetic elements, such as primers to composite systems designed for gene expression in Escherichia coli (E. coli), Saccharomyces cerevisiae (S. cerevisiae) and Bacillus subtilis (B. subtilis).
Each part has been designed for project Ash Guard, however they represent versatile genetic components and optimization methodology applied to synthetic biology.
Explore each part below and on the Parts Registry!
| Part ID | Part Name | Description |
|---|---|---|
| BBa_K5103000 | Cry8Da with PCG004 unique BsaI cut sites | Their part contains Cry8Da, which encodes a protein known to act as an insecticidal toxin specifically targeting members of the Coleoptera order, including the emerald ash borer (EAB). The Cry8Da gene is flanked by BsaI restriction sites, which allow for easy integration into a variety of plasmid backbones. |
| BBa_K5103001 | PCG004 with Cry8Da | This is a composite part that includes pCG004, a shuttle plasmid backbone designed for Escherichia coli (E. coli), with the Cry8Da gene inserted. The Cry8Da gene is under the control of an IPTG-inducible promoter, enabling regulation of protein expression. This composite part has been demonstrated to work effectively in E. coli DH5α, and the expression of Cry8Da is regulated using the IPTG-inducible promoter. This system can be used for producing the Cry8Da protein in E. coli for laboratory studies, protein purification, and potential applications in bioinsecticide development. |
| BBa_K5103002 | pYES2 plasmid backbone cut with HindIII and XhoI | This part represents the pYES2 plasmid, a yeast expression system that has been cut with the restriction enzymes HindIII and XhoI. These cut sites enable the insertion of foreign genes, such as Cry8Da, for expression in Saccharomyces cerevisiae (S. cerevisiae). The pYES2 plasmid is a synthetic yeast expression vector with the GAL1 promoter, commonly used for galactose-inducible expression in S. cerevisiae. |
| BBa_K5103003 | Cry8Da with HindIII and XhoI cut sites introduced using primers | This part represents the Cry8Da gene cut with HindIII and XhoI restriction enzymes. These cut sites enable the gene to be inserted into expression vectors like pYES2 for yeast expression. |
| BBa_K5103004 | Cry8Da Cloned in pYES2 Yeast Plasmid | This part represents the pYES2 plasmid with the Cry8Da gene inserted. The gene is under the control of the GAL1 promoter, allowing inducible expression in S. cerevisiae when galactose is present. |
| BBa_K5103005 | PCG004 plasmid cut with BsaI for cloning Cry8Da | This part represents the pGC004 plasmid cut with the BsaI restriction enzyme, allowing for the integration of the Cry8Da gene. pCG004 is a commonly used plasmid developed for high efficiency cloning and expression in E. coli and Bacillus subtilis. |
| BBa_K5103006 | Forward primer introducing a HindIII cut site to the 5’ end of Cry8Da | This part is a forward primer designed to introduce a HindIII restriction site at the 5’ end of the Cry8Da gene. This primer allows for the directional cloning of Cry8Da into expression plasmids that utilize the HindIII site for gene insertion. This primer is intended for use in systems where HindIII cut sites are necessary for cloning Cry8Da into plasmid backbones such as pYES2 for yeast. |
| BBa_K5103007 | Reverse primer introducing a XhoI cut site to the 3’ end of Cry8Da | This part is a reverse primer designed to introduce a XhoI restriction site at the 3’ end of the Cry8Da gene. This primer facilitates the directional cloning of Cry8Da into expression vectors that use XhoI sites for gene insertion. This primer is used to clone Cry8Da into plasmids that rely on XhoI cut sites for gene insertion, such as pYES2. |