of global dengue cases
cases of dengue fever
mosquito-borne diseases
However, drawbacks of these chemicals include:
To perform L-Borneol Biosynthesis (LBB), we conducted Gibson assembly on our plasmid fragments to form our pACYC-LBB and pBAD-LBB plasmids.
Then, the completed pACYC-LBB and pBAD-LBB plasmids were transformed into the DH5α strain of E. coli using heat shock, facilitating the diffusion of plasmids into the DH5α E. coli.
The transformed E. coli BLR (DE3) is allowed to clone itself in an incubator to make millions of copies of our engineered plasmids.
We added Isopropyl β-D-1-thiogalactopyranoside (IPTG) and arabinose to induce protein production in transformed bacteria. This allowed the rapid production of all proteins in the production pathway, resulting in the production of L-Borneol.
