This year, our team developed and refined a diverse array of genetic
parts, each tailored for specific functions within our project. These
components were carefully curated and aligned with the BioBricks
assembly standard, ensuring compatibility and ease of use. On this page,
we provide a concise overview of our parts, organized by the modules
they contribute to in our system. For in-depth insights and technical
details, we encourage you to explore the relevant sections on The iGEM
Parts Registry or check out our Design and Engineering page for further
information.
Basic Parts
| BBa_K5241001 |
Pahdd |
Encodes 4-hydroxyphenylpyruvate dioxygenase, involved in melanin
synthesis. |
| BBa_K5241002 |
Pkrhdd |
Encodes 4-hydroxyphenylpyruvate dioxygenase. |
| BBa_K5241003 |
Tyrosinase(BM) |
Tyrosinase that converts tyrosine to dopamine and dopamine to
dopaquinone. |
| BBa_K5241004 |
mel |
The mel gene encodes tyrosinase, which is one of the key enzymes in
melanin synthesis. |
Composite Parts
| BBa_K5241005 |
Pahdd-6xH in pET-21b(+) |
T7 promoter lac operator RBS T7 tag Pahdd 6xHis T7 terminator |
| BBa_K5241006 |
pBAD-6xH-pelBPkrhdd-6xH |
Encodes a fusion protein consisting of the Pkrhdd enzyme from
Pseudomonas koreensis, an N-terminal T7 tag for solubility, and a
C-terminal 6xHis tag for purification. The fusion protein is under the
control of the pBAD promoter, which allows for arabinose-inducible
expression, and confers ampicillin resistance (AmpR). |
| BBa_K5241007 |
PelB-PkhppD-6xH-20b（+） |
Based on Pseudomonas_koreensis_D26
[gene=hppD][protein=4-hydroxyphenylpyruvate dioxygenase], a gene with a
PelB signal peptide and 6His tag is synthesized, aiming for the
tyrosinase to be localized to the bacterial periplasmic space. |
| BBa_K5241008 |
pPICZaAPkrhdd-6xH |
“PICZα” refers to a vector containing the yeast alpha-factor signal
peptide sequence, which aids in the proper secretion of proteins. It was
developed by Invitrogen (now part of Thermo Fisher Scientific).
“pPICZαA-PHDD-6xH” is a vector used for expressing the PHDD protein with
a 6xHis tag in Pichia pastoris. |
| BBa_K5241009 |
GAL1 promoter-Tyrosinase(BM) -CYC1 terminator |
In brewing yeast, the GAL1 promoter, tyrosine as the target gene,
and the CYC1 terminator are used to express the tyrosine synthesis
pathway for the production of melanin in yeast. |
| BBa_K5241010 |
GAL1-mel-CYC1 |
In Pseudomonas maltophilia, the mel gene encodes tyrosinase, which
is one of the key enzymes in melanin synthesis. This enzyme catalyzes
the conversion of tyrosine to L-DOPA and ultimately forms melanin
through a series of oxidation reactions. |
| BBa_K5241011 |
ADE2-gal1-egfp-CYC1 |
The ADE2 gene is combined with the yeast gal1 promoter, the enhanced
green fluorescent protein gene egfp, and the CYC1 terminator. |
