In this page, our team presents the parts generated during our project. We develop an in-cell assay to investigate the functionality of the human hormone receptors in S. cerevisiae as well as a cell-free system to investigate EDCs in the water. During our project, we designed 5 basic parts as well as 6 composite parts which consist of our new Biobricks as well as previously submitted parts in the iGEM repository. BBa_K5107007 is our candidate for the best composite part. If you are already familiar with our project click on the Parts ID and navigate to the iGEM repository.

Basic Parts

Parts ID	Name	Description
BBa_K5107000	HRE minimal	The consensus sequence for the binding site of the human Androgen Receptor (AR), Glucocorticoid Receptor (GR), Mineralocorticoid Receptor (MR), and Progesterone receptor (PR).
BBa_K5107001	ERE minimal	The consensus sequence for the binding site of the human Estrogen Receptor α (ERα) and Estrogen Receptor β (ERβ).
BBa_K5107009	6xHIS-hAR	Open reading frame (ORF) of human Androgen receptor (hAR) for expresssion and purification in yeast.
BBa_K5107010	hAR/pRR	The human Androgen Receptor coding region utilized for our in-cell biosensor.
BBa_K5107013	hGR/pRR	The human Glucocorticoid Receptor coding region utilized for our in-cell biosensor.

Composite Parts

Parts ID	Name	Description
BBa_K5107002	HRE5	HRE5 is the five tandem repeats of the HRE minimal to increase the interaction surface of the hormone receptor with the DNA.
BBa_K5107004	ERE5	ERE5 is the five tandem repeats of the ERE minimal to increase the interaction surface of the hormone receptor with the DNA.
BBa_K5107006	T7-HRE5-dB-T	T7-HRE5-dB-T is a construct used in the cell-free biosensor. The HRE5 is recognised by the steroid androgen, progesterone and glucocorticoid hormone receptors. Through this interaction, the in vitro transcription of a dimeric broccoli aptamer is controlled based on the presence of EDCs.
BBa_K5107007	T7-HREmin-sB-T	T7-HREminimal-sB-T is a construct used in the cell free biosensor. The HREminimal is recognised by the steroid androgen, progesterone and glucocorticoid hormone receptors. Through this interaction, the in vitro transcription of a monomeric broccoli aptamer is controlled based on the presence of EDCs.
BBa_K5107008	T7-EREmin-sB-T	T7-EREminimal-sB-T is a construct used in the cell free biosensor.The EREmininimal is recognised by the steroid estrogen hormone receptor. Through this interaction, the in vitro transcription of a monomeric broccoli aptamer is controlled based on the presence of EDCs.
BBa_K5107012	T7-ERE5-dB-T	T7-ERE5-dB-T is a construct used in the cell free biosensor. The ERE5 is recognised by the steroid estrogen hormone receptor. Through this interaction, the in vitro transcription of a dimeric broccoli aptamer is controlled based on the presence of EDCs.