Summary
As a member of BIT-China, an old IGEM team, we have always put safety in the first place. Every member of our team has undergone systematic laboratory safety training and has a high safety awareness. Especially for the students in the experimental group, after 24 class hours of training on laboratory safety and experimental standard operation taught by the instructor, we finally selected qualified team members to carry out the experiment in groups under the leadership of senior graduate students after the examination of written test and practical operation. Among them, the advisors are all graduate students with rich experimental experience who have obtained the Laboratory Safety Training Certificate after passing the school and college exams. The college also provided us with a simulation experience platform for learning laboratory safety operation precautions and emergency prevention and control measures to ensure that the team members fully focus on experimental safety.
Laboratory description
The laboratory supported by the BIT-China team is the Key Laboratory of the Ministry of Pharmaceutical Molecular Science and Pharmaceutical Engineering Industry and Information Technology, which has extremely detailed regulations on laboratory safety. For experimental instruments, real-name reservation system and examination system are used to standardize, which minimizes a series of laboratory safety hazards caused by improper operation of experimental instruments.
Laboratory Safety
Wear white coat (required), gloves, mask, glasses, etc.; Wear a mask during weighing and live bacteria handling experiments.
Do not wear slippers, sandals, short sleeves, shorts and other clothes that expose the skin directly to the experiment, so as to avoid sputtering acid, alkali, toxic substances, etc.
Do not wear loose hair, tie long hair to avoid being burned or falling into the reagent, do not wear high heels.
No headphones.
The last student to leave the lab checks that the instruments are turned off and locks the doors and Windows.
Mark your own experimental materials well (anyone has the right to dispose of unmarked experimental materials, material name, owner, date).
Don't take other people's lab material.
Don't make loud noises in the lab.
Do not eat in the experimental area.
Do not wear gloves to touch things outside the experimental area.
Improve the sensitivity to abnormal sounds and tastes in the laboratory, so as to find hidden dangers in time and eliminate them in the bud.
Laboratory Waste Management.
Bacteria solution and bacteria can not be discharged directly.
Put the needle into a special container.
Solid liquid waste separation on the bench.
Discard gloves and masks in the designated place.
Preventing leakage of engineered bacteria
We exchanged our project design with Prof. Chun Li and Prof. Huan Sun (see Human Practice page for more details) and received valuable guidance. Since our project involves microbial production, we need to strictly control the spread of microorganisms into the environment, and we have designed a set of careful control measures for this purpose:
We will use a closed bioreactor for microbial culture.
All operators will be required to use an alcohol program after conducting experiments.
We will periodically disinfect all culture equipment and materials with UV light and autoclaving.
Waste disposal processes will be established to ensure that microorganisms are not transmitted through waste.
First Aid
Know the location of the laboratory in the building.
First aid kit.
Eye wash and safe shower location.
Fire extinguisher location.
Fire escape route.
119 and the security office telephone.
If you cut or bleed during the experimental operation, rinse with running water first, disinfect with 75% alcohol, disinfect with iodophor in the first aid kit if necessary, and bandage with gauze or band-aid.
If the cutting tool is iron, especially rusty iron, also need to see a doctor tetanus vaccine.
