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Table.1 Basic Parts

Name Function and Discription
BBa_K4737004 GOx a gene encodes Glucose oxidase (GOx) from Aspergillus niger
BBa_K5166000 Nickel-Binding Peptide 1 a Nickel-binding peptide which can bind Ni(Ⅱ) specificaly
BBa_K5166001 Nickel-Binding Peptide 2 a Nickel-binding peptide which can bind Ni(Ⅱ) specificaly
BBa_K5166002 Nickel-Binding Peptide 3 a Nickel-binding peptide which can bind Ni(Ⅱ) specificaly
BBa_K5166003 Nickel-Binding Peptide 4 a Nickel-binding peptide which can bind Ni(Ⅱ) specificaly
BBa_K5166004 Cobalt-Binding Peptide 1 a Cobalt-binding peptide which can bind Co(Ⅱ) specificaly
BBa_K5166005 Cobalt-Binding Peptide 2 a Cobalt-binding peptide which can bind Co(Ⅱ) specificaly
BBa_K5166006 Lithium-Binding Peptide 3 a Lithium-binding peptide which can bind Li(Ⅰ) specificaly
BBa_K5166007 mntR a Manganese-binding protein which can bind Mn(Ⅱ) specificaly
BBa_K5166008 TssS a Manganese-binding protein which can bind Mn(Ⅱ) specificaly
BBa_K5166009 BH2807 a Manganese-binding protein which can bind Mn(Ⅱ) specificaly
BBa_K5166010 GAP promoter a Manganese-binding protein which can bind Mn(Ⅱ) specificaly
BBa_K5166011 α-factor secretion signal a constitutive promoter
BBa_K5166012 pir1p a signalling that guide protein secretion outside the cell
BBa_K5166013 Myc a anchoring proteins
BBa_K5166014 AOX1 terminator a tag to detect the amount of protein expression
BBa_K5166030 linker a terminator used in P. pastoris
BBa_K5166015 UreA a short DNA that link between target protein and pir
BBa_K5166016 UreB Functional subunit of urease,can form a heterotrimer with UreB (β) and UreC (α) subunits,which is already active urease
BBa_K5166017 UreC Functional subunit of urease,can form a heterotrimer with UreA(γ) and UreC (α) subunits,which is already active urease
BBa_K5166018 UreE Functional subunit of urease,can form a heterotrimer with UreA(γ) and UreB (β) subunits,which is already active urease
BBa_K5166019 UreF Accessory factor of urease,can form a complex with UreF and UreG,which acts as a GTP hydrolysis-dependent molecular chaperone
BBa_K5166020 UreG Accessory factor of urease,can form a complex with UreE and UreG,which acts as a GTP hydrolysis-dependent molecular chaperone
BBa_K5166021 UreD Accessory factor of urease,can form a complex with UreE and UreF,which acts as a GTP hydrolysis-dependent molecular chaperone
BBa_K5166022 UreA RBS1 Accessory factor of urease,a metal-binding chaperone that aids in the provision of nickel
BBa_K5166023 UreB RBS2 RBS of UreA
BBa_K5166024 UreC RBS3 RBS of UreB
BBa_K5166025 UreE RBS4 RBS of UreC
BBa_K5166026 UreF RBS5 RBS of UreE
BBa_K5166027 UreG RBS6 RBS of UreF
BBa_K5166028 UreD RBS7 RBS of UreG
BBa_K5166029 T7-Lac operator promoter RBS of UreD
BBa_B0014 Double terminator 2 Terminator of coding UreEFGD
BBa_B0015 Double terminator 1 Terminator of coding UreABC

Table.2 Composite Parts

Name Function and Discription
BBa_K5166032 Pichia Pastoris GS115 Surface Display System a yeast surface display system
BBa_K5166033 pGAPZα-NBP1-pir a yeast surface display system containing NBP1
BBa_K5166034 pGAPZα-NBP2-pir a yeast surface display system containing NBP2
BBa_K5166035 pGAPZα-NBP3-pir a yeast surface display system containing NBP3
BBa_K5166036 pGAPZα-NBP4-pir a yeast surface display system containing NBP4
BBa_K5166067 pGAPZα-CPB1-pir a yeast surface display system containing CBP1
BBa_K5166038 pGAPZα-CPB2-pir a yeast surface display system containing CBP2
BBa_K5166039 pGAPZα-LBP3-pir a yeast surface display system containing LBP3
BBa_K5166040 pGAPZα-mntR-pir a yeast surface display system containing mntR
BBa_K5166041 pGAPZα-TssS-pir a yeast surface display system containing TssS
BBa_K5166042 pGAPZα-BH2807-pir a yeast surface display system containing BH2807
BBa_K5166066 UreA-UreB-UreC UreABC is the primary functional subunit of bacterial Urease protein,they combine to form a hetero-trimer, which binds together to create an active enzyme.When co-expressed with UreEFGD (the accessory subunit) from another part, it can produce bacterial Urease.
BBa_K5166065 UreE-UreF-UreG-UreD UreEFGD serves as the accessory factor subunit for bacterial urease proteins. When UreABC, which forms the catalytic heterotrimer, is co-expressed with the accessory subunit UreEFGD, it leads to the production of functional bacterial urease.
BBa_K4665005 SazCA SazCA surface expression system