The goal of our project was to test the Jungle Express inducible-repressible system in methanotrophic bacteria and see whether or not it could prove to be a powerful new inducible system for bioengineering. The goal of inducible systems is to allow the scientist to precisley control expression of their desired downstream prodcut. But most current systems are leaky, and show a base level of expression of the desired product. The paper where the Jungle Express system was originally identified stated that there was no basal expression detected at all when no inducing molecule was added, meaning that this system has the potential to confer much tighter control in experimental organisms like e. coli and methanotrophs. The Jungle Express system is essentially an operator (EilR) that binds to specific sequences by the -10 and -35 region of a promoter to prevent RNA Polymerase from binding and intiating transcription. The operator, EilR can be induced using crystal violet, which is a cationic inorganic dye that interacts with the active site in EilR with three key residues. Crystal violet is cheap and likely won't be mistaken for any molecule that exists in the media an organism is grown in, or any molecule an organism may produce naturally through its own metabolism meaning that the chances of an incorrect molecule inducing expression is incredibly low, granting a higher level of control and expression than is seen in typical inducible system. The binding site for the operator is preceeded in the paper by a few frequently used phage promoters, and in our project we used those as well as two novel promoters pulled from methanotrophs and added the operator binding sites to test whether or not the system would work when incorporated into a native promoter not tested within the paper. If we could prove that there was no base level expression, we would use the system to express a few subunits of the methane monooxygenase enzyme in e. coli. If there was any expression at all without induction, the e. coli would not grow becuase the subunits are proven to be toxic. If there was no expression until it was induced we would be able to express the subunits in e. coli and isolate them for further screening and structure elucidation.