Experiments Calendar
May 30, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Zihan Jiang]:
Sterilizing tips and pipettes. Prepare the LB broth. Writing experimental protocols.
May 31, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Zihan Jiang]:
Bacteria incubation in 100 ml LB broth overnight (1358, 1362, 1364, and 1833). Writing experimental protocols.
Jun 1, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Zihan Jiang]:
Conduct the plasmids midi-purification and DNA concentrations are as below. 1362 and 1833 are qualified for our transfection experiments.
Jun 2, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Zihan Jiang]:
Find the protocols of medium preparation, cell culture and passage. Prepare the trypsin/EDTA, PBS, FBS, p.s., milli-Q water and sterilize the bottles.
Jun 3, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Gigi Lei, Yuan Tang]:
DMEM preparation and get the HEK293T cells.
Jun 4, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Zihan Jiang]:
Waiting for the HEK293T cells to grow. Search and rewrite the experiment protocols of transfection with HEK293T cells (lentivirus packaging).
Jun 5, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Zihan Jiang]:
Conduct the transfection with HEK293T cells (1108, 1356 and 1833).
Jun 6, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Zihan Jiang]:
Change the medium of the transfected cells. HEK293T cell passage.
Jun 7, 2024 [Daoing MA, Zihan Jiang, Zihan Jiang]:
Lentivirus collection. Change the fresh medium to cells
Jun 8, 2024 [Daoming MA, Zixing Chen]:
Lentivirus collection. Change the fresh medium to cells.
Jun 10, 2024 [Daoming Ma, Zixing Chen, Zihan Jiang]:
Prepare LB broth for more plasmid extraction. HEK293T cell passage.
Jun 11, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Conduct plasmid midi-purification. The color of the bacteria culture during midi-prep is too turbid, the suggestion given is that we should pick a single bacteria colony and use that for further amplification.
Jun 12, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Prepare the LB agar and incubate bacteria on them.
Jun 13, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Gigi Lei, QianYu Gu]:
Select the single colon bacteria and incubate in 100 ml LB broth for amplification.
HEK293T cell culture and passage to the six-well plates and 10 cm dish.
Jun 14, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, QianYu Gu, Zihan Jiang]:
Plasmids midi-prep.
Jun 15, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Gigi Lei]:
HEK293T cell culture and passage.
Jun 16, 2024 [Daoming MA, Zixing Chen, Gigi Lei]:
HEK293T cell infection to generate the stable cell lines with lentivirus (1833 and 1362).
Jun 18, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Gigi Lei, SiCheng Zhou]:
HEK293T cell culture. Change the medium in six-well plates to the fresh one with G418.
Jun 19, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, SiCheng Zhou]:
Transfection with HEK293T cells (1108 and 1358).
Jun 20, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Gigi Lei]:
Change the medium in six-well plates to the fresh one with G418. HEK293T cell culture. Change the medium in the transfected cells to the refresh one.
Jun 21, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Gigi Lei]:
Change the medium in six-well plates to the fresh one with G418. Collect the lentivirus.
Jun 22, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Change the medium in six-well plates to the fresh one with G418. Collect the lentivirus.
Jun 24, 2024 [Daoming MA, Gigi Lei]:
Prepare the LB broth and incubate the bacterias in 100 ml LB broth overnight for amplification.
Jun 25, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Gigi Lei]:
Conduct plasmids midi-prep.
Jun 25, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Qianyu Gu]:
Change the medium in six-well plates to the fresh one with G418. HEK293T cell culture.
Jun 26, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Qianyu Gu]:
Fluorescence microscope detection (1833 success, and 1362 fail).
Jun 28, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Qianyu Gu]:
Run PCR to check the DNA.
Jun 29, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Qianyu Gu]:
HEK293T cell culture and passage.
Jul 2, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Qianyu Gu]:
Transfection and Infection.
Jul 3, 2024 [DaoMing MA, Zixing Chen, Zhe Zhang, Qianyu Gu]:
HEK293T cell culture and passage.
Jul 4, 2024 [Daoming MA, Zixing Chen, Qianyu Gu]:
Cell cytometry for cell sorting. (the sorted cell number is too small, and we wait for the cell to grow for about 10 days.)
Jul 5, 2024 [Daoming MA, Zixing Chen, Qianyu Gu]:
Prepare the LB broth and incubate the bacteria in 100 ml LB broth overnight.
Jul 6, 2024 [Daoming MA, Zixing Chen, Qianyu Gu]:
Conduct the midi-prep.
Jul 7, 2024 [Daoming MA]:
HEK293T cell culture and passage.
Jul 8, 2024 [Daoming MA, Zixing Chen, Gigi Lei]:
Lentivirus collection, cell passage.
Jul 9-15, 2024 [Zixing Chen, Zhe Zhang, Gigi Lei]:
HEK293T cell culture and passage. Transfection and infection.
Jul 17, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Stable HEK293T cell lines freezing.
Jul 18, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Change the medium to the Opti-MEM (reduced serum medium) for the exosomes extraction.
Jul 20, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Qianyu Gu]:
Collect the supernatant containing the exosomes and conduct the exosomes extraction experiments.
Jul 22, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Yaolin Shi]:
HEK 293T cell transfection, cell infection, and fluorescent imaging.
Jul 23, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Yaolin Shi]:
Conduct the exosomes extraction experiments.
Jul 24, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Yaolin Shi]:
Run PCR.
Jul 27, 2024 [Daoming Ma, Yaolin Shi]:
HEK293T cell culture and passage.
Jul 28, 2024 [Daoming Ma, Yaolin Shi]:
Prepare the LB broth and incubate the bacteria in 100 ml LB broth overnight for amplification.
Jul 29, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Yaolin Shi]:
Plasmid midi-prep.
Jul 30, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Sicheng Zhou]:
1362, 1358, 1108, 1356 cell transfection.
Jul 31, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Sicheng Zhou]:
DMEM medium preparation.
Aug 1, 2024 [Daoming MA, Sicheng Zhou]:
DLS measurement and PCR.
Aug 2, 2024 [Daoming MA, Zixing Chen, Zhe Zhang, Sicheng Zhou]:
HEK293T cell culture and passage.
Aug 4, 2024 [Daoming MA, Sicheng Zhou]:
HEK293T cell transfection and infection.
Aug 6, 2024 [Zixing Chen, Zhe Zhang, Sicheng Zhou]:
Lentivirus collection.
Aug 7, 2024 [Zhe Zhang, Sicheng Zhou]:
Lentivirus collection and HEK293T cell culture.
Aug 9, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
HEk293T cell culture and passage.
Aug 11, 2024 [Daoming MA, Sicheng Zhou]:
HEK293T cell culture and fluorescence microscope imaging.
Aug 12, 2024 [Zhe Zhang]:
Conduct the exosomes extraction experiments.
Aug 13, 2024 [Zhe Zhang, Sicheng Zhou]:
HEK293T cell culture and passage.
Aug 15, 2024 [Zixing Chen, Sicheng Zhou]:
HEK293T cell culture and apotome fluorescence microscope imaging.
Aug 16, 2024[Zhe Zhang, Sicheng Zhou]:
Send the exosomes to do the TEM imaging (one week). HEK293T cell culture and passage.
Aug 19, 2024[Daoming MA, Zixing Chen, Zhe Zhang]:
RPMI1640 medium preparation.
Aug 20, 2024 [Daoming MA, Sicheng Zhou]:
Cancer cell culture.
Aug 21, 2024 [Zixing Chen, Zhe Zhang]:
BCA assay. Cancer cer culture and passage.
Aug 22, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Western blot.
Aug 23, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Western blot. Cancer cell culture and passage.
Aug 25, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Exosome extration.
Aug 26, 2024 [Daoming MA, Sicheng Zhou]:
DLS measurement.
Aug 27, 2024 [Daoming MA, Zhe Zhang, Sicheng Zhou]:
Ultrasound stimulation.
Aug 28, 2024 [Zixing Chen, Zhe Zhang]:
Cancer cell apoptosis assay.
Aug 28, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Cancer cell apoptosis assay.
Aug 30, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Meeting with professor Yehuda Assaraf
Sept 1, 2024 [Daoming MA, Sicheng Zhou]:
Cancer cell proliferation assay.
Sept 3, 2024 [Daoming Ma, Zhe Zhang]:
Cell cytometry.
Sept 4, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Cancer cell culture and passage.
Sept 9, 2024 [Daoming MA, Zhe Zhang, Sicheng Zhou]:
BCA assay and western blot.
Sept 10, 2024 [[Daoming MA, Zixing Chen, Sicheng Zhou]:
Western blot.
Sept 12, 2024 [Zhe Zhang, Zixing Chen]:
Exosome extraction.
Sept 15, 2024 [Zixing Chen, Sicheng Zhou]:
Infect the cancer cells directly with the lentivirus.
Sept 16, 2024 [Daoming MA]:
Apotome flurescence microscope.
Sept 17, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Ultrasound stimulation.
Sept 18, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Cancer cell apoptosis assay.
Sept 19, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Cancer cell proliferation assay.
Sept 20, 2024 [Daoming MA, Zixing Chen, Zhe Zhang]:
Exosome extraction and DLS measurement.
