Notebook
7.15-7.19 pET-PBAD-StayGold
7.18-7.24 Construction of plasmid PET-28a-CsnB
7.24-7.30 Construction of plasmid PET-28a-CDA and CsnB mutant
7.31-8.05 Expression, enzyme activity measurement and product analysis of chitin deacetylase CDA
8.05-8.08 Expression, enzyme activity assay and product analysis of CsnB and its mutants
8.09-8.16 CDA and CsnB mutants synergistically catalyze the synthesis of chitosan from chitin