iGEM overview
Read our logbook, depicting our whole iGEM journey.
Wetlab notebooks
Wetlab overview
Read the overview about all our wetlab activities.
Domestication of gBlocks
Domestication of gBlocks: Learn about how we cloned the gBlocks in plasmid as a preparation for the operon assembly and enzyme production for characterization.
Pyrene-Operon-Assembly
Pyrene-Operon-Assembly: See how we assembled the operon BBa_K5043008 with all genes needed for the pyrene degradation.
Enzyme characterization
Enzyme characterization: Read about our process of characterizing the pdoA2B2-phtAcAd enzyme complex.
Pseudomonas cultivation with PAHs
Pseudomonas cultivation with PAHs: See how we produced data showing how Pseudomonas putida and Pseudomonas vancouverensis deal with exposure to PAHs.
Immobilization
Immobilization: Read to understand how we got to our preferred immobilization methods.