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Safety

Our laboratory work is conducted at lab B515-A（Molecular biology laboratory）of Zhejiang University. The laboratory is a Biosafety Level 1 (BSL-1)laboratory, under the responsibility of Dr. Dong Shan. The processed material does not cause disease in humans and most animals. Our experiment is conducted under the supervision of professional teachers. We utilized common bacterial E. coli TOP10 and Rosetta to ensure the safety of our project.

Our team took all the necessary precautions to ensure we do not cause any environmental or personal harm.

Fig1

Lab Safety One​

Prior to starting any actual experiments, the lab teacher let us sign the Summer Experiment Safety Informed Consent Form. We make sure that our lab were followed the lab-safety guidelines and all equipment was used with caution.After the experiment is completed, glassware should be disinfected with alcohol, then cleaned with detergent, and finally rinsed with ultrapure water. Chemical waste should be disposed of in biohazard waste containers; chemicals and glassware should be sterilized in an autoclave. The acid stains 4s green plus nucleic acid stain is relatively safe.

Fig2

Product​

The product itself is a bacterial expression product, primarily consisting of enzymes that participate in the subsequent reaction, and these enzymes are safe.

The product manufactured in the lab and not released into the environment.The engineered E. coli strain is non-pathogenic and does not produce endotoxins, making it relatively safe for use in biopharmaceuticals.

Fig3

Value​

NAOS has good moisture absorption, moisturizing and whitening effect. It has good antioxidant activity and can clear up ⋅\cdotO2- and ⋅\cdotOH free radicals.

Laboratory Safety Management Regulations​

In order to ensure the safety of all personnel and experimental equipment in the laboratory, to prevent accidents caused by human factors, and to avoid personal injury and property loss, the following safety management standards are hereby formulated. All staff entering the laboratory must study carefully and comply with them.

I. General Provision​

Firmly establish the awareness of three safety responsibilities (responsible for personal safety, responsible for the safety of colleagues in the laboratory, and responsible for the safety of laboratory equipment) and the awareness of biological laboratory safety to ensure the safe operation of the laboratory.

II. Personal Safety Protection​

  1. When working in the laboratory, one should wear a laboratory coat, and it must be taken off and left inside the laboratory when leaving. In principle, one should not wear a laboratory coat into public places.

  2. Disposable gloves should be worn when hands may come into contact with infectious materials, contaminated surfaces, or equipment, and gloves should only be removed after the work is completely finished. During and after the experimental operation with gloves, gloves should not touch one's own or others' exposed skin, as well as non-experimental items such as water cups and mobile phones. Gloves should not be worn out of the laboratory or touch public areas that others may touch, such as door handles and elevator buttons. Disposable gloves should be discarded promptly after removal and should not be washed and reused.

  3. Goggles should be worn when the experimental process may involve splashing liquids or other flying objects.

  4. When the experimental process involves toxic and harmful gases or volatile liquids, the operation should be carried out in a fume hood.

III.Experimental Equipment, Experimental Consumables, and Experimental Operations​

  1. Care for all kinds of instruments and equipment in the laboratory, and do not misuse or damage them. Those who damage the instruments and equipment due to subjective intention or violation of regulations should compensate according to the price.

  2. Use all kinds of experimental consumables correctly, and do not misuse or waste them. Those who cause abnormal consumption of consumables due to subjective intention or violation of regulations, if the situation is serious, should compensate according to the price.

  3. Before starting the experiment, one must clarify the experimental objectives and requirements, master the basic principles, and be familiar with the operational steps. Before operating the experimental instruments and equipment, one must understand the performance of the experimental instruments, the methods of operation and use, and the precautions.

  4. During the experimental process, one must follow the guidance of the teacher and is not allowed to operate without permission.

IV.Other Matters Needing Attention​

  1. All instruments, equipment, and various items in the laboratory must not be taken out of the room privately. If any damage or loss of instruments and equipment occurs, it should be reported to the teacher immediately. If there is concealment and failure to report, once found, double compensation is required.

  2. Strictly abide by the experimental discipline, and do not make noise, chase, or play in the laboratory. The use of instruments and drugs unrelated to the experiment is prohibited.

  3. Respect the intellectual property rights and work results of others. Without permission, one should not look through, photograph, or operate others' experimental records and materials, nor should one operate others' experimental materials or instruments and equipment that are in use.

  4. Eating, smoking, cleaning contact lenses, and applying makeup are strictly prohibited in the laboratory.

  5. After the experiment, one must check the instruments, ensure that the instruments are neatly arranged, and perform cleaning work before leaving the laboratory.

  6. Before leaving the laboratory, one should close the doors and windows, and put the waste paper and other debris generated during the experiment into the laboratory's trash can or take it away.