Organizing campus promotions for iGEM 2024.

Holding a biology contest to select team members for NUDT-CHINA 2024.

Establishment of team NUDT-CHINA 2024. Team member chose their interesting groups according to their interests and advantages, including Wet Lab, Human Practice, Art&Design.

Conducting a literature review on industries associated with insomnia.

Conducting a questionnaire survey among over 2,000 participants.

Laboratory safety training.

Brainstorming for 2024 team project.

Learning the molecular biology and basic experimental techniques.

Determining the initial direction of the 2024 iGEM project, considering to choose Melatonin as our fundamental molecule to design our platform. However, the core circuits to detect remained undecided.

Determining three circuits as the directions to design the platform for the detection of melatonin receptor agonists. Till now, our little knight had been ready for training to protect our sleep.

Conducting preliminary Proof of Concept design and plasmid design.

Start constructing MTNR1A plasmid with normal molecular strategy.

Start constructing NFAT plasmid with normal molecular strategy.

The NFAT plasmid was co-transfected with MTNR1A plasmid and MTNR1A-5xNFAT engineered cells were constructed by cis-transfection and assayed with melatonin.

Changing the multiplier, 1xNFAT, 6xNFAT and 7xNFAT corresponding engineered cells were constructed and assayed.

Development of the 1st generation engineered cell.

Detection of changes in intracellular calcium ion concentration in engineered cells using GCaMP plasmid transfected cells.

Start constructing CRE plasmid with normal molecular strategy.

The CRE plasmid was co-transfected with MTNR1A plasmid and MTNR1A-4xCRE engineered cells were constructed by cis-transfection and assayed with melatonin.

Construction of MTNR1A-4xCRE-cAMP stabilized transient cell line

Changing the multiplier, 5xCRE and 6xCRE corresponding engineered cells were constructed and assayed.

Screening of plant essential oils using MTNR1A-4xCRE-cAMP stable lines.

Molecular docking using MTNR1A-4xCRE-cAMP stable lines.

Production of the Promotion Video.

Creation of team giveaways and mascots.

Offline meeting of the wet lab group for further designing and engineering.

Start of production for the Science Popularization Picture Book.