Attributions

Tasks:

Conceptualization, Writing, Safety, Public Engagement, Entrepreneurship, Data Curation

Specific tasks:

(1) Analysis - Analyze data from different sources; (2) Project Management - Manage the activities, planning and execution of the entire project; (3) Writing - Participate in writing all parts of the wiki and check the text written by your partner. (4) Organize scientific knowledge dissemination activities in primary and secondary schools in the underdeveloped Northwest region to address educational inequalities and remove barriers to the dissemination of biological and health science knowledge (5) Organize a series of community outreach activities, such as lectures, interactive experiences and questionnaires, to integrate type 2 diabetes prevention knowledge into the community and create a collective atmosphere for the prevention of type 2 diabetes in the whole society. (6) Microscope slide painting activities are particularly important in modern society, and the popularization of biological science and health knowledge. To do this, we held a unique event – painting on a slide. The event not only showcased the artistic talents of the participants, but also aimed to promote knowledge related to biology and chronic diseases, as well as promote public awareness and understanding of health issues. (8) Held an event with the theme of "LZU-MEDICINE-CHINA Creates a Biomedical Film Journey". LANZHOU UNIVERSITY STUDENTS WERE INVITED TO VISIT OUR AFFILIATED HOSPITAL, WHERE THEY DETAILED THE REMARKABLE ACHIEVEMENTS AND EXCITING PROJECTS THAT OUR LZU-MEDICINE-CHINA TEAM HAS ACHIEVED OVER THE YEARS. Students visited the laboratory and performed simple experiments such as DNA extraction and agarose gel electrophoresis under the guidance of their supervisors.

Tasks:

Conceptualization, Writing, Safety, Public Engagement

Specific tasks:

(1) Analytics - Analyze data from different sources; (2) Conceptualization - Propose plans and details for the implementation of the project; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (4) Writing - Participate in writing all parts of the wiki. (5) Organize scientific knowledge dissemination activities in primary and secondary schools in the underdeveloped northwest region to solve the problem of educational inequality and remove barriers to the dissemination of biological and health science knowledge (6) Organize a series of community outreach activities, such as lectures, interactive experiences and questionnaires, etc., to integrate type 2 diabetes prevention knowledge into the community and create a collective atmosphere for the prevention of type 2 diabetes in the whole society. ( 7 ) Invite students from the main campus of Lanzhou University to visit our team's laboratory, so that they have the opportunity to experience the charm of biology up close. (8) Carry out the "Lanzhou University Yuzhong Campus Science Quiz and Lucky Draw Activity" In response to the World Health Organization's call for popular science education to improve students' understanding of synthetic biology and type 2 diabetes, a popular science answer lottery was carefully planned. This event is not only a feast of knowledge, but also a journey of popular science.

Tasks:

Conceptualization, Writing, Visualization, Public Engagement, Entrepreneurship, Project Administration

Specific tasks:

(1) Analytics - Analyze data from different sources; (2) Conceptualization - Propose plans and details for the implementation of the project; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (4) Writing - Participate in writing all parts of the wiki. (5) Organize scientific knowledge dissemination activities in primary and secondary schools in the underdeveloped northwest region to solve the problem of educational inequality and remove barriers to the dissemination of biological and health science knowledge (6) Organize a series of community outreach activities, such as lectures, interactive experiences and questionnaires, etc., to integrate type 2 diabetes prevention knowledge into the community and create a collective atmosphere for the prevention of type 2 diabetes in the whole society. ( 7 ) Invite students from the main campus of Lanzhou University to visit our team's laboratory, so that they have the opportunity to experience the charm of biology up close. (8) Carry out the "Lanzhou University Yuzhong Campus Science Quiz and Lucky Draw Activity" In response to the World Health Organization's call for popular science education to improve students' understanding of synthetic biology and type 2 diabetes, a popular science answer lottery was carefully planned. This event is not only a feast of knowledge, but also a journey of popular science.

Tasks:

Conceptualization, Writing, Safety, Public Engagement, Project Administration, Entrepreneurship

Specific tasks:

(1) Analysis - Analyze data from different sources; (2) Project Management - Manage the activities, planning and execution of the entire project; (3) Writing - Participate in writing all parts of the wiki and check the text written by your partner. (4) Organize scientific knowledge dissemination activities in primary and secondary schools in the underdeveloped Northwest region to address educational inequalities and remove barriers to the dissemination of biological and health science knowledge (5) Organize a series of community outreach activities, such as lectures, interactive experiences and questionnaires, to integrate type 2 diabetes prevention knowledge into the community and create a collective atmosphere for the prevention of type 2 diabetes in the whole society. (6) Microscope slide painting activities are particularly important in modern society, and the popularization of biological science and health knowledge. To do this, we held a unique event – painting on a slide. The event not only showcased the artistic talents of the participants, but also aimed to promote knowledge related to biology and chronic diseases, as well as promote public awareness and understanding of health issues. (8) Held an event with the theme of "LZU-MEDICINE-CHINA Creates a Biomedical Film Journey". LANZHOU UNIVERSITY STUDENTS WERE INVITED TO VISIT OUR AFFILIATED HOSPITAL, WHERE THEY DETAILED THE REMARKABLE ACHIEVEMENTS AND EXCITING PROJECTS THAT OUR LZU-MEDICINE-CHINA TEAM HAS ACHIEVED OVER THE YEARS. Students visited the laboratory and performed simple experiments such as DNA extraction and agarose gel electrophoresis under the guidance of their supervisors.

Tasks:

Conceptualization, Writing, Public Engagement, Visualization, Entrepreneurship

Specific tasks:

(1) Analytics - Analyze data from different sources; (2) Conceptualization - Propose plans and details for the implementation of the project; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (4) Writing - Participate in writing all parts of the wiki. (5) Organize scientific knowledge dissemination activities in primary and secondary schools in the underdeveloped northwest region to solve the problem of educational inequality and remove barriers to the dissemination of biological and health science knowledge (6) Organize a series of community outreach activities, such as lectures, interactive experiences and questionnaires, etc., to integrate type 2 diabetes prevention knowledge into the community and create a collective atmosphere for the prevention of type 2 diabetes in the whole society. ( 7 ) Invite students from the main campus of Lanzhou University to visit our team's laboratory, so that they have the opportunity to experience the charm of biology up close. (8) Carry out the "Lanzhou University Yuzhong Campus Science Quiz and Lucky Draw Activity" In response to the World Health Organization's call for popular science education to improve students' understanding of synthetic biology and type 2 diabetes, a popular science answer lottery was carefully planned. This event is not only a feast of knowledge, but also a journey of popular science.

Tasks:

Conceptualization, Public Engagement, Writing, Entrepreneurship, Safety, Visualization

Specific tasks:

(1) Analysis - Analyze data from different sources; (2) Project Management - Manage the activities, planning and execution of the entire project; (3) Writing - Participate in writing all parts of the wiki and check the text written by your partner. (4) Organize scientific knowledge dissemination activities in primary and secondary schools in the underdeveloped Northwest region to address educational inequalities and remove barriers to the dissemination of biological and health science knowledge (5) Organize a series of community outreach activities, such as lectures, interactive experiences and questionnaires, to integrate type 2 diabetes prevention knowledge into the community and create a collective atmosphere for the prevention of type 2 diabetes in the whole society. (6) Microscope slide painting activities are particularly important in modern society, and the popularization of biological science and health knowledge. To do this, we held a unique event – painting on a slide. The event not only showcased the artistic talents of the participants, but also aimed to promote knowledge related to biology and chronic diseases, as well as promote public awareness and understanding of health issues. (8) Held an event with the theme of "LZU-MEDICINE-CHINA Creates a Biomedical Film Journey". LANZHOU UNIVERSITY STUDENTS WERE INVITED TO VISIT OUR AFFILIATED HOSPITAL, WHERE THEY DETAILED THE REMARKABLE ACHIEVEMENTS AND EXCITING PROJECTS THAT OUR LZU-MEDICINE-CHINA TEAM HAS ACHIEVED OVER THE YEARS. Students visited the laboratory and performed simple experiments such as DNA extraction and agarose gel electrophoresis under the guidance of their supervisors.

Tasks:

Analysis, Background Research, Investigation, Public Engagement, Visualization, Data Curation

Specific tasks:

Coach and assist team members in the following: (1) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (2) Invite students from the main campus of Lanzhou University to visit our team's laboratory, so that they have the opportunity to experience the charm of biology up close. (3) HOLD AN EVENT WITH THE THEME OF "LZU-MEDICINE-CHINA CREATES A BIOMEDICAL FILM JOURNEY". LANZHOU UNIVERSITY STUDENTS WERE INVITED TO VISIT OUR AFFILIATED HOSPITAL, WHERE THEY DETAILED THE REMARKABLE ACHIEVEMENTS AND EXCITING PROJECTS THAT OUR LZU-MEDICINE-CHINA TEAM HAS ACHIEVED OVER THE YEARS. Students visited the laboratory and performed simple experiments such as DNA extraction and agarose gel electrophoresis under the guidance of their supervisors. (4) In response to the call of the World Health Organization for popular science education, and to improve students' understanding of synthetic biology and type 2 diabetes, a popular science answer lottery was carefully planned. This event is not only a feast of knowledge, but also a journey of popular science. Through interactive Q&A, we aim to spark students' interest in cutting-edge science, especially in the emerging field of synthetic biology, and in the prevention and treatment of type 2 diabetes.

Tasks:

Background Research, Data Curation, Fundraising, Writing

Specific tasks:

Coach and assist team members in the following: (1) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (2) Invite students from the main campus of Lanzhou University to visit our team's laboratory, so that they have the opportunity to experience the charm of biology up close. (3) HOLD AN EVENT WITH THE THEME OF "LZU-MEDICINE-CHINA CREATES A BIOMEDICAL FILM JOURNEY". LANZHOU UNIVERSITY STUDENTS WERE INVITED TO VISIT OUR AFFILIATED HOSPITAL, WHERE THEY DETAILED THE REMARKABLE ACHIEVEMENTS AND EXCITING PROJECTS THAT OUR LZU-MEDICINE-CHINA TEAM HAS ACHIEVED OVER THE YEARS. Students visited the laboratory and performed simple experiments such as DNA extraction and agarose gel electrophoresis under the guidance of their supervisors. (4) In response to the call of the World Health Organization for popular science education, and to improve students' understanding of synthetic biology and type 2 diabetes, a popular science answer lottery was carefully planned. This event is not only a feast of knowledge, but also a journey of popular science. Through interactive Q&A, we aim to spark students' interest in cutting-edge science, especially in the emerging field of synthetic biology, and in the prevention and treatment of type 2 diabetes.

Tasks:

Background Research, Public Engagement, Visualization, Fundraising, Safety, Writing

Specific tasks:

Coach and assist team members in the following: (1) In order to stimulate the public's interest in medicine and improve medical students' understanding of anatomy and art, an online anatomy and art drawing activity was planned - online anatomy and art painting activity. Combining medical knowledge with artistic creation, this event aims to foster a strong interest in anatomy among medical students, while allowing the public to understand medical knowledge in a more acceptable way. (2) In order to become a disseminator of health knowledge, aiming to spread the light of science to every corner of the community, especially for the current growing problem of type 2 diabetes, we bring a vivid and practical health lecture to the residents of the Gaolan Road community. (3) Organize a series of community outreach activities, such as lectures, interactive experiences and questionnaires, to integrate the knowledge of type 2 diabetes prevention into the community and create a collective atmosphere for the prevention of type 2 diabetes in the whole society. (4) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (5) Organize scientific knowledge dissemination activities in primary and secondary schools in the underdeveloped northwest region to solve the problem of educational inequality and remove barriers to the dissemination of biological and health science knowledge

Tasks:

Entrepreneurship, Analysis, Investigation, Conceptualization, Data Curation, Project Administration, Wiki Coding

Specific tasks:

Coach and assist team members in the following: (1) In order to stimulate the public's interest in medicine and improve medical students' understanding of anatomy and art, an online anatomy and art drawing activity was planned - online anatomy and art painting activity. Combining medical knowledge with artistic creation, this event aims to foster a strong interest in anatomy among medical students, while allowing the public to understand medical knowledge in a more acceptable way. (2) In order to become a disseminator of health knowledge, aiming to spread the light of science to every corner of the community, especially for the current growing problem of type 2 diabetes, we bring a vivid and practical health lecture to the residents of the Gaolan Road community. (3) Organize a series of community outreach activities, such as lectures, interactive experiences and questionnaires, to integrate the knowledge of type 2 diabetes prevention into the community and create a collective atmosphere for the prevention of type 2 diabetes in the whole society. (4) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (5) Organize scientific knowledge dissemination activities in primary and secondary schools in the underdeveloped northwest region to solve the problem of educational inequality and remove barriers to the dissemination of biological and health science knowledge

Tasks:

Data Curation, Notebook/record keeping, Background Research, Investigation, Project Administration, Writing

Specific tasks:

Coach and assist team members in the following: Through glucose concentration gradient plate experiments, the compatibility of chassis bacteria EcN with the experiment was verified and evaluated. In order to simulate different blood glucose concentrations, L B. Agar plate: L. without glucose B. Agarose agar plate, L. containing 2.0 mmol/L glucose B. Agar plates, L. agar plates containing 5.0 mmol/L glucose, L-B agar plates containing 8.0 mmol/L glucose, and L-B agar plates containing 11.0 mmol/L glucose are used to represent the low, normal, and high blood glucose ranges. Subsequently, these agar plates were inoculated with the same dilution factor of chassis ECN1917 and engineering bacteria containing introduced plasmids to evaluate their growth status. The results showed that all types of bacteria grew normally on various plates, and there was no statistically significant difference in colony counts.

Tasks:

Analysis, Background Research, Investigation, Data Curation, Notebook/record keeping, Writing, Safety

Specific tasks:

Coach and assist team members in the following: Through glucose concentration gradient plate experiments, the compatibility of chassis bacteria EcN with the experiment was verified and evaluated. In order to simulate different blood glucose concentrations, L B. Agar plate: L. without glucose B. Agarose agar plate, L. containing 2.0 mmol/L glucose B. Agar plates, L. agar plates containing 5.0 mmol/L glucose, L-B agar plates containing 8.0 mmol/L glucose, and L-B agar plates containing 11.0 mmol/L glucose are used to represent the low, normal, and high blood glucose ranges. Subsequently, these agar plates were inoculated with the same dilution factor of chassis ECN1917 and engineering bacteria containing introduced plasmids to evaluate their growth status. The results showed that all types of bacteria grew normally on various plates, and there was no statistically significant difference in colony counts.

Tasks:

Conceptualization, Writing, Public Engagement, Visualization

Specific tasks:

Coach and assist team members in the following: We designed self-assembled nanocapsules based on 1,2-distearoyl-sn-glycerin-3-phosphoethanolamine (DLPC) to prevent potential exposure of immunogenic components and division of engineered bacteria. 1,2-distearoyl-sn-glycerin-3-phosphoethanolamine polyethylene glycol (DSPE-PEG) is used as the main component of the membrane, and cholesterol (1% w/w) is added to improve the stability of the membrane. Calcium ion solution is used to promote the self-assembly of engineering bacteria surface facial mask. Afterwards, we diluted and inoculated conventional engineering bacteria and engineering bacteria loaded in nanocapsules onto the agar plates used in the first iteration to investigate whether nanocapsules inhibit bacterial division. In addition, we also used DMAO/PI staining to determine whether the engineered bacteria maintained normal survival rates. Proving the inhibitory effect of vesicles on the division of engineering bacteria. Afterwards, normal engineering bacteria and bacteria encapsulated in nanocapsules were cultured at the same concentration in PBS buffer with the highest glucose concentration (1+B51.0 mmol/L), aiming to inhibit the proliferation of normal engineering bacteria caused by the lack of exogenous amino acids and nucleotides. To detect the secretion of SCFA and GLP-1 recombinant proteins, we used high-performance liquid chromatography (HPLC) and GLP-1 ELISA kit to measure the levels of target substances in the buffer. The results indicate that both normal engineering bacteria and engineering bacteria encapsulated in nanocapsules can produce target products. However, the production of GLP-1 is 53.7% of that of normal bacteria. This indicates that although GLP-1 recombinant protein can pass through phospholipid complex vesicles, its diffusion rate per unit time is relatively low.

Tasks:

Analysis, Conceptualization, Background Research, Investigation, Notebook/record keeping, Writing, Visualization, Safety

Specific tasks:

Coach and assist team members in the following: We designed self-assembled nanocapsules based on 1,2-distearoyl-sn-glycerin-3-phosphoethanolamine (DLPC) to prevent potential exposure of immunogenic components and division of engineered bacteria. 1,2-distearoyl-sn-glycerin-3-phosphoethanolamine polyethylene glycol (DSPE-PEG) is used as the main component of the membrane, and cholesterol (1% w/w) is added to improve the stability of the membrane. Calcium ion solution is used to promote the self-assembly of engineering bacteria surface facial mask. Afterwards, we diluted and inoculated conventional engineering bacteria and engineering bacteria loaded in nanocapsules onto the agar plates used in the first iteration to investigate whether nanocapsules inhibit bacterial division. In addition, we also used DMAO/PI staining to determine whether the engineered bacteria maintained normal survival rates. Proving the inhibitory effect of vesicles on the division of engineering bacteria. Afterwards, normal engineering bacteria and bacteria encapsulated in nanocapsules were cultured at the same concentration in PBS buffer with the highest glucose concentration (1+B51.0 mmol/L), aiming to inhibit the proliferation of normal engineering bacteria caused by the lack of exogenous amino acids and nucleotides. To detect the secretion of SCFA and GLP-1 recombinant proteins, we used high-performance liquid chromatography (HPLC) and GLP-1 ELISA kit to measure the levels of target substances in the buffer. The results indicate that both normal engineering bacteria and engineering bacteria encapsulated in nanocapsules can produce target products. However, the production of GLP-1 is 53.7% of that of normal bacteria. This indicates that although GLP-1 recombinant protein can pass through phospholipid complex vesicles, its diffusion rate per unit time is relatively low.

Tasks:

Analysis, Conceptualization, Data Curation, Investigation, Safety, Background Research, Hardware, Writing

Specific tasks:

Coach and assist team members in the following: Due to the presence of kanamycin resistance genes in the recombinant plasmid, we will dilute the bacterial suspension using an electroporation device and inoculate it into kanamycin L B. Select resistant colonies on agar plates. Afterwards, we will select individual colonies for amplification, extract total RNA according to the requirements of the total RNA extraction kit, and use designed primers for real-time quantitative PCR to verify whether the recombinant plasmid has been successfully introduced into the engineered bacteria. The results showed that chassis bacteria without plasmids did not grow on kanamycin plates, while engineered bacteria formed colonies on kanamycin plates. The PCR results confirmed that the plasmid had been successfully introduced into the engineered bacteria. Based on the above experimental results, we further validated the protein expression. Based on existing literature and databases, we estimate that the molecular weight of the BCoAT protein transcribed from the pET28a BCoAT His plasmid should be around 46 kDa. We will treat the engineered bacteria at the highest glucose concentration L B. Cultivate in liquid medium for 8 hours to reach the logarithmic growth stage, then use an ultrasonic cell disruptor to lyse bacteria on ice and centrifuge at high speed to obtain cell-free products. SDS-PAGE gel electrophoresis was used for protein separation, and His antibody was used for specific detection. We have successfully isolated the BCoAT protein, and its molecular weight is consistent with the expected value. Based on the above findings, we will further validate the expression of the second target protein. We are at the highest glucose concentration in L B. Cultivate the engineered bacteria in liquid medium for 8 hours to reach the logarithmic growth stage, and then collect the supernatant by high-speed centrifugation. Measure protein concentration using BCA protein quantification kit and separate proteins through SDS-PAGE. Subsequently, we used FLAG labeled antibodies for specific Western blot screening to capture the target protein. The results of SDS-PAGE gel electrophoresis and Western blot showed that the recombinant GLP-1 protein was indeed expressed in the supernatant of the engineered bacteria, which confirmed that it was successfully secreted into the extracellular space.

Tasks:

Project Administration, Investigation, Analysis, Data Curation, Fundraising, Hardware, Background Research, Safety

Specific tasks:

Coach and assist team members in the following: Due to the presence of kanamycin resistance genes in the recombinant plasmid, we will dilute the bacterial suspension using an electroporation device and inoculate it into kanamycin L B. Select resistant colonies on agar plates. Afterwards, we will select individual colonies for amplification, extract total RNA according to the requirements of the total RNA extraction kit, and use designed primers for real-time quantitative PCR to verify whether the recombinant plasmid has been successfully introduced into the engineered bacteria. The results showed that chassis bacteria without plasmids did not grow on kanamycin plates, while engineered bacteria formed colonies on kanamycin plates. The PCR results confirmed that the plasmid had been successfully introduced into the engineered bacteria. Based on the above experimental results, we further validated the protein expression. Based on existing literature and databases, we estimate that the molecular weight of the BCoAT protein transcribed from the pET28a BCoAT His plasmid should be around 46 kDa. We will treat the engineered bacteria at the highest glucose concentration L B. Cultivate in liquid medium for 8 hours to reach the logarithmic growth stage, then use an ultrasonic cell disruptor to lyse bacteria on ice and centrifuge at high speed to obtain cell-free products. SDS-PAGE gel electrophoresis was used for protein separation, and His antibody was used for specific detection. We have successfully isolated the BCoAT protein, and its molecular weight is consistent with the expected value. Based on the above findings, we will further validate the expression of the second target protein. We are at the highest glucose concentration in L B. Cultivate the engineered bacteria in liquid medium for 8 hours to reach the logarithmic growth stage, and then collect the supernatant by high-speed centrifugation. Measure protein concentration using BCA protein quantification kit and separate proteins through SDS-PAGE. Subsequently, we used FLAG labeled antibodies for specific Western blot screening to capture the target protein. The results of SDS-PAGE gel electrophoresis and Western blot showed that the recombinant GLP-1 protein was indeed expressed in the supernatant of the engineered bacteria, which confirmed that it was successfully secreted into the extracellular space.

Tasks:

Analysis, Background Research, Investigation, Public Engagement, Writing, Data Curation

Specific tasks:

Coach and assist team members in the following: Due to the presence of kanamycin resistance genes in the recombinant plasmid, we will dilute the bacterial suspension using an electroporation device and inoculate it into kanamycin L B. Select resistant colonies on agar plates. Afterwards, we will select individual colonies for amplification, extract total RNA according to the requirements of the total RNA extraction kit, and use designed primers for real-time quantitative PCR to verify whether the recombinant plasmid has been successfully introduced into the engineered bacteria. The results showed that chassis bacteria without plasmids did not grow on kanamycin plates, while engineered bacteria formed colonies on kanamycin plates. The PCR results confirmed that the plasmid had been successfully introduced into the engineered bacteria. Based on the above experimental results, we further validated the protein expression. Based on existing literature and databases, we estimate that the molecular weight of the BCoAT protein transcribed from the pET28a BCoAT His plasmid should be around 46 kDa. We will treat the engineered bacteria at the highest glucose concentration L B. Cultivate in liquid medium for 8 hours to reach the logarithmic growth stage, then use an ultrasonic cell disruptor to lyse bacteria on ice and centrifuge at high speed to obtain cell-free products. SDS-PAGE gel electrophoresis was used for protein separation, and His antibody was used for specific detection. We have successfully isolated the BCoAT protein, and its molecular weight is consistent with the expected value. Based on the above findings, we will further validate the expression of the second target protein. We are at the highest glucose concentration in L B. Cultivate the engineered bacteria in liquid medium for 8 hours to reach the logarithmic growth stage, and then collect the supernatant by high-speed centrifugation. Measure protein concentration using BCA protein quantification kit and separate proteins through SDS-PAGE. Subsequently, we used FLAG labeled antibodies for specific Western blot screening to capture the target protein. The results of SDS-PAGE gel electrophoresis and Western blot showed that the recombinant GLP-1 protein was indeed expressed in the supernatant of the engineered bacteria, which confirmed that it was successfully secreted into the extracellular space.

Tasks:

Data Curation, Background Research, Conceptualization, Investigation, Notebook/record keeping, Other, Project Administration, Analysis

Specific tasks:

Design experimental procedures and guide the entire experimental process. Due to the presence of kanamycin resistance genes in the recombinant plasmid, we will dilute the bacterial suspension using an electroporation device and inoculate it into kanamycin L B. Select resistant colonies on agar plates. Afterwards, we will select individual colonies for amplification, extract total RNA according to the requirements of the total RNA extraction kit, and use designed primers for real-time quantitative PCR to verify whether the recombinant plasmid has been successfully introduced into the engineered bacteria. The results showed that chassis bacteria without plasmids did not grow on kanamycin plates, while engineered bacteria formed colonies on kanamycin plates. The PCR results confirmed that the plasmid had been successfully introduced into the engineered bacteria. Based on the above experimental results, we further validated the protein expression. Based on existing literature and databases, we estimate that the molecular weight of the BCoAT protein transcribed from the pET28a BCoAT His plasmid should be around 46 kDa. We will treat the engineered bacteria at the highest glucose concentration L B. Cultivate in liquid medium for 8 hours to reach the logarithmic growth stage, then use an ultrasonic cell disruptor to lyse bacteria on ice and centrifuge at high speed to obtain cell-free products. SDS-PAGE gel electrophoresis was used for protein separation, and His antibody was used for specific detection. We have successfully isolated the BCoAT protein, and its molecular weight is consistent with the expected value. Based on the above findings, we will further validate the expression of the second target protein. We are at the highest glucose concentration in L B. Cultivate the engineered bacteria in liquid medium for 8 hours to reach the logarithmic growth stage, and then collect the supernatant by high-speed centrifugation. Measure protein concentration using BCA protein quantification kit and separate proteins through SDS-PAGE. Subsequently, we used FLAG labeled antibodies for specific Western blot screening to capture the target protein. The results of SDS-PAGE gel electrophoresis and Western blot showed that the recombinant GLP-1 protein was indeed expressed in the supernatant of the engineered bacteria, which confirmed that it was successfully secreted into the extracellular space.

Tasks:

Analysis, Investigation, Background Research, Data Curation, Conceptualization, Notebook/record keeping, Other, Project Administration

Specific tasks:

Design experimental procedures and guide the entire experimental process. Used high-performance liquid chromatography (HPLC) to measure the expression of three SCFAs (acetic acid, propionic acid, and butyric acid) produced by engineering bacteria under time-series conditions, and validated the ability of engineering bacteria to release GLP-1 through glucose concentration gradient experiments. The results indicate that compared to chassis bacteria, engineering bacteria are capable of producing all three types of short chain fatty acids (SCFAs). This confirms that the BCoAT gene operates normally in engineered strains. Time series analysis showed that after 12 hours of inoculation into the new culture medium, the concentrations of the three SCFAs gradually increased, corresponding to the transition of the engineered bacteria from the logarithmic growth stage to the stable stage. Afterwards, we established a glucose concentration gradient in the culture medium and a time series at the highest glucose concentration (simulating a hyperglycemic environment) to cultivate the engineered bacteria. Afterwards, we collected the supernatant from the culture by centrifugation and measured the concentration of GLP-1 in the supernatant using ELISA to infer its release results. The results showed that under the same cultivation time conditions, the concentration of GLP-1 in the supernatant increased with the increase of glucose content in the culture medium. In the time series measurement of the highest glucose concentration (11mmol/L), GLP-1 concentration gradually increased within 12 hours and then stabilized.

Tasks:

Analysis, Conceptualization, Investigation, Background Research, Data Curation, Notebook/record keeping, Other

Specific tasks:

Through glucose concentration gradient plate experiments, the compatibility of chassis bacteria EcN with the experiment was verified and evaluated. In order to simulate different blood glucose concentrations, L B. Agar plate: L. without glucose B. Agarose agar plate, L. containing 2.0 mmol/L glucose B. Agar plates, L. agar plates containing 5.0 mmol/L glucose, L-B agar plates containing 8.0 mmol/L glucose, and L-B agar plates containing 11.0 mmol/L glucose are used to represent the low, normal, and high blood glucose ranges. Subsequently, these agar plates were inoculated with the same dilution factor of chassis ECN1917 and engineering bacteria containing introduced plasmids to evaluate their growth status. The results showed that all types of bacteria grew normally on various plates, and there was no statistically significant difference in colony counts.

Tasks:

Analysis, Conceptualization, Investigation, Background Research, Notebook/record keeping, Data Curation, Other

Specific tasks:

We designed self-assembled nanocapsules based on 1,2-distearoyl-sn-glycerin-3-phosphoethanolamine (DLPC) to prevent potential exposure of immunogenic components and division of engineered bacteria. 1,2-distearoyl-sn-glycerin-3-phosphoethanolamine polyethylene glycol (DSPE-PEG) is used as the main component of the membrane, and cholesterol (1% w/w) is added to improve the stability of the membrane. Calcium ion solution is used to promote the self-assembly of engineering bacteria surface facial mask. Afterwards, we diluted and inoculated conventional engineering bacteria and engineering bacteria loaded in nanocapsules onto the agar plates used in the first iteration to investigate whether nanocapsules inhibit bacterial division. In addition, we also used DMAO/PI staining to determine whether the engineered bacteria maintained normal survival rates. Proving the inhibitory effect of vesicles on the division of engineering bacteria. Afterwards, normal engineering bacteria and bacteria encapsulated in nanocapsules were cultured at the same concentration in PBS buffer with the highest glucose concentration (1+B51.0 mmol/L), aiming to inhibit the proliferation of normal engineering bacteria caused by the lack of exogenous amino acids and nucleotides. To detect the secretion of SCFA and GLP-1 recombinant proteins, we used high-performance liquid chromatography (HPLC) and GLP-1 ELISA kit to measure the levels of target substances in the buffer. The results indicate that both normal engineering bacteria and engineering bacteria encapsulated in nanocapsules can produce target products. However, the production of GLP-1 is 53.7% of that of normal bacteria. This indicates that although GLP-1 recombinant protein can pass through phospholipid complex vesicles, its diffusion rate per unit time is relatively low.

Tasks:

Analysis, Conceptualization, Investigation, Background Research, Data Curation, Notebook/record keeping, Other

Specific tasks:

We designed self-assembled nanocapsules based on 1,2-distearoyl-sn-glycerin-3-phosphoethanolamine (DLPC) to prevent potential exposure of immunogenic components and division of engineered bacteria. 1,2-distearoyl-sn-glycerin-3-phosphoethanolamine polyethylene glycol (DSPE-PEG) is used as the main component of the membrane, and cholesterol (1% w/w) is added to improve the stability of the membrane. Calcium ion solution is used to promote the self-assembly of engineering bacteria surface facial mask. Afterwards, we diluted and inoculated conventional engineering bacteria and engineering bacteria loaded in nanocapsules onto the agar plates used in the first iteration to investigate whether nanocapsules inhibit bacterial division. In addition, we also used DMAO/PI staining to determine whether the engineered bacteria maintained normal survival rates. Proving the inhibitory effect of vesicles on the division of engineering bacteria. Afterwards, normal engineering bacteria and bacteria encapsulated in nanocapsules were cultured at the same concentration in PBS buffer with the highest glucose concentration (1+B51.0 mmol/L), aiming to inhibit the proliferation of normal engineering bacteria caused by the lack of exogenous amino acids and nucleotides. To detect the secretion of SCFA and GLP-1 recombinant proteins, we used high-performance liquid chromatography (HPLC) and GLP-1 ELISA kit to measure the levels of target substances in the buffer. The results indicate that both normal engineering bacteria and engineering bacteria encapsulated in nanocapsules can produce target products. However, the production of GLP-1 is 53.7% of that of normal bacteria. This indicates that although GLP-1 recombinant protein can pass through phospholipid complex vesicles, its diffusion rate per unit time is relatively low.

Tasks:

Analysis, Background Research, Data Curation, Notebook/record keeping, Investigation, Conceptualization, Other

Specific tasks:

Due to the presence of kanamycin resistance genes in the recombinant plasmid, we will dilute the bacterial suspension using an electroporation device and inoculate it into kanamycin L B. Select resistant colonies on agar plates. Afterwards, we will select individual colonies for amplification, extract total RNA according to the requirements of the total RNA extraction kit, and use designed primers for real-time quantitative PCR to verify whether the recombinant plasmid has been successfully introduced into the engineered bacteria. The results showed that chassis bacteria without plasmids did not grow on kanamycin plates, while engineered bacteria formed colonies on kanamycin plates. The PCR results confirmed that the plasmid had been successfully introduced into the engineered bacteria. Based on the above experimental results, we further validated the protein expression. Based on existing literature and databases, we estimate that the molecular weight of the BCoAT protein transcribed from the pET28a BCoAT His plasmid should be around 46 kDa. We will treat the engineered bacteria at the highest glucose concentration L B. Cultivate in liquid medium for 8 hours to reach the logarithmic growth stage, then use an ultrasonic cell disruptor to lyse bacteria on ice and centrifuge at high speed to obtain cell-free products. SDS-PAGE gel electrophoresis was used for protein separation, and His antibody was used for specific detection. We have successfully isolated the BCoAT protein, and its molecular weight is consistent with the expected value. Based on the above findings, we will further validate the expression of the second target protein. We are at the highest glucose concentration in L B. Cultivate the engineered bacteria in liquid medium for 8 hours to reach the logarithmic growth stage, and then collect the supernatant by high-speed centrifugation. Measure protein concentration using BCA protein quantification kit and separate proteins through SDS-PAGE. Subsequently, we used FLAG labeled antibodies for specific Western blot screening to capture the target protein. The results of SDS-PAGE gel electrophoresis and Western blot showed that the recombinant GLP-1 protein was indeed expressed in the supernatant of the engineered bacteria, which confirmed that it was successfully secreted into the extracellular space.

Tasks:

Analysis, Background Research, Conceptualization, Investigation, Data Curation, Notebook/record keeping, Other

Specific tasks:

Due to the presence of kanamycin resistance genes in the recombinant plasmid, we will dilute the bacterial suspension using an electroporation device and inoculate it into kanamycin L B. Select resistant colonies on agar plates. Afterwards, we will select individual colonies for amplification, extract total RNA according to the requirements of the total RNA extraction kit, and use designed primers for real-time quantitative PCR to verify whether the recombinant plasmid has been successfully introduced into the engineered bacteria. The results showed that chassis bacteria without plasmids did not grow on kanamycin plates, while engineered bacteria formed colonies on kanamycin plates. The PCR results confirmed that the plasmid had been successfully introduced into the engineered bacteria. Based on the above experimental results, we further validated the protein expression. Based on existing literature and databases, we estimate that the molecular weight of the BCoAT protein transcribed from the pET28a BCoAT His plasmid should be around 46 kDa. We will treat the engineered bacteria at the highest glucose concentration L B. Cultivate in liquid medium for 8 hours to reach the logarithmic growth stage, then use an ultrasonic cell disruptor to lyse bacteria on ice and centrifuge at high speed to obtain cell-free products. SDS-PAGE gel electrophoresis was used for protein separation, and His antibody was used for specific detection. We have successfully isolated the BCoAT protein, and its molecular weight is consistent with the expected value. Based on the above findings, we will further validate the expression of the second target protein. We are at the highest glucose concentration in L B. Cultivate the engineered bacteria in liquid medium for 8 hours to reach the logarithmic growth stage, and then collect the supernatant by high-speed centrifugation. Measure protein concentration using BCA protein quantification kit and separate proteins through SDS-PAGE. Subsequently, we used FLAG labeled antibodies for specific Western blot screening to capture the target protein. The results of SDS-PAGE gel electrophoresis and Western blot showed that the recombinant GLP-1 protein was indeed expressed in the supernatant of the engineered bacteria, which confirmed that it was successfully secreted into the extracellular space.

Tasks:

Analysis, Background Research, Data Curation, Notebook/record keeping, Investigation, Conceptualization, Other

Specific tasks:

We used high-performance liquid chromatography (HPLC) to measure the expression of three SCFAs (acetic acid, propionic acid, and butyric acid) produced by engineering bacteria under time-series conditions, and validated the ability of engineering bacteria to release GLP-1 through glucose concentration gradient experiments. The results indicate that compared to chassis bacteria, engineering bacteria are capable of producing all three types of short chain fatty acids (SCFAs). This confirms that the BCoAT gene operates normally in engineered strains. Time series analysis showed that after 12 hours of inoculation into the new culture medium, the concentrations of the three SCFAs gradually increased, corresponding to the transition of the engineered bacteria from the logarithmic growth stage to the stable stage. Afterwards, we established a glucose concentration gradient in the culture medium and a time series at the highest glucose concentration (simulating a hyperglycemic environment) to cultivate the engineered bacteria. Afterwards, we collected the supernatant from the culture by centrifugation and measured the concentration of GLP-1 in the supernatant using ELISA to infer its release results. The results showed that under the same cultivation time conditions, the concentration of GLP-1 in the supernatant increased with the increase of glucose content in the culture medium. In the time series measurement of the highest glucose concentration (11mmol/L), GLP-1 concentration gradually increased within 12 hours and then stabilized.

Tasks:

Analysis, Investigation, Background Research, Data Curation, Notebook/record keeping, Conceptualization, Other

Specific tasks:

We used high-performance liquid chromatography (HPLC) to measure the expression of three SCFAs (acetic acid, propionic acid, and butyric acid) produced by engineering bacteria under time-series conditions, and validated the ability of engineering bacteria to release GLP-1 through glucose concentration gradient experiments. The results indicate that compared to chassis bacteria, engineering bacteria are capable of producing all three types of short chain fatty acids (SCFAs). This confirms that the BCoAT gene operates normally in engineered strains. Time series analysis showed that after 12 hours of inoculation into the new culture medium, the concentrations of the three SCFAs gradually increased, corresponding to the transition of the engineered bacteria from the logarithmic growth stage to the stable stage. Afterwards, we established a glucose concentration gradient in the culture medium and a time series at the highest glucose concentration (simulating a hyperglycemic environment) to cultivate the engineered bacteria. Afterwards, we collected the supernatant from the culture by centrifugation and measured the concentration of GLP-1 in the supernatant using ELISA to infer its release results. The results showed that under the same cultivation time conditions, the concentration of GLP-1 in the supernatant increased with the increase of glucose content in the culture medium. In the time series measurement of the highest glucose concentration (11mmol/L), GLP-1 concentration gradually increased within 12 hours and then stabilized.

Tasks:

Fundraising, Conceptualization, Data Curation, Visualization, Safety

Specific tasks:

Coach and assist team members in the following: We used high-performance liquid chromatography (HPLC) to measure the expression of three SCFAs (acetic acid, propionic acid, and butyric acid) produced by engineering bacteria under time-series conditions, and validated the ability of engineering bacteria to release GLP-1 through glucose concentration gradient experiments. The results indicate that compared to chassis bacteria, engineering bacteria are capable of producing all three types of short chain fatty acids (SCFAs). This confirms that the BCoAT gene operates normally in engineered strains. Time series analysis showed that after 12 hours of inoculation into the new culture medium, the concentrations of the three SCFAs gradually increased, corresponding to the transition of the engineered bacteria from the logarithmic growth stage to the stable stage. Afterwards, we established a glucose concentration gradient in the culture medium and a time series at the highest glucose concentration (simulating a hyperglycemic environment) to cultivate the engineered bacteria. Afterwards, we collected the supernatant from the culture by centrifugation and measured the concentration of GLP-1 in the supernatant using ELISA to infer its release results. The results showed that under the same cultivation time conditions, the concentration of GLP-1 in the supernatant increased with the increase of glucose content in the culture medium. In the time series measurement of the highest glucose concentration (11mmol/L), GLP-1 concentration gradually increased within 12 hours and then stabilized.

Tasks:

Hardware, Conceptualization, Fundraising, Safety

Specific tasks:

(1) Instruct the team to complete background checks, design experiments and project preparation. (2) Provide corresponding technical support for the smooth development and completion of the project, such as guiding the corresponding experimental operation and result analysis and processing. (3) Provide corresponding financial support for the smooth development and completion of this project, such as providing the purchase of bacterial and cell samples and related reagents required for the experiment. (4) Provide corresponding laboratory and experimental equipment support for the wet experimental work of this project. (5) Provide corresponding on-site support for the project, carry out project discussions, regular meetings and other activities.

Tasks:

Conceptualization, Safety, Fundraising

Specific tasks:

(1) Instruct the team to complete background checks, design experiments and project preparation. (2) Provide corresponding technical support for the smooth development and completion of the project, such as guiding the corresponding experimental operation and result analysis and processing. (3) Provide corresponding financial support for the smooth development and completion of this project, such as providing the purchase of bacterial and cell samples and related reagents required for the experiment. (4) Provide corresponding laboratory and experimental equipment support for the wet experimental work of this project. (5) Provide corresponding on-site support for the project, carry out project discussions, regular meetings and other activities.

Tasks:

Software, Conceptualization, Investigation, Background Research, Data Curation, Safety

Specific tasks:

Coach and assist team members in the following: We used high-performance liquid chromatography (HPLC) to measure the expression of three SCFAs (acetic acid, propionic acid, and butyric acid) produced by engineering bacteria under time-series conditions, and validated the ability of engineering bacteria to release GLP-1 through glucose concentration gradient experiments. The results indicate that compared to chassis bacteria, engineering bacteria are capable of producing all three types of short chain fatty acids (SCFAs). This confirms that the BCoAT gene operates normally in engineered strains. Time series analysis showed that after 12 hours of inoculation into the new culture medium, the concentrations of the three SCFAs gradually increased, corresponding to the transition of the engineered bacteria from the logarithmic growth stage to the stable stage. Afterwards, we established a glucose concentration gradient in the culture medium and a time series at the highest glucose concentration (simulating a hyperglycemic environment) to cultivate the engineered bacteria. Afterwards, we collected the supernatant from the culture by centrifugation and measured the concentration of GLP-1 in the supernatant using ELISA to infer its release results. The results showed that under the same cultivation time conditions, the concentration of GLP-1 in the supernatant increased with the increase of glucose content in the culture medium. In the time series measurement of the highest glucose concentration (11mmol/L), GLP-1 concentration gradually increased within 12 hours and then stabilized.

Tasks:

Other, Safety

Specific tasks:

Provided comprehensive and detailed guidance for the development of a series of experiments, taking into account various factors and potential challenges that may arise during the experimental process. This guidance not only included the scientific and technical aspects such as experimental design, methodology selection, and data analysis, but also emphasized the importance of ensuring safety throughout the entire experimental procedure. Safety measures were carefully formulated and implemented to protect the researchers, the experimental environment, and the integrity of the experiments. Regular safety training sessions were organized to familiarize the research team with safety protocols and emergency response procedures. Appropriate safety equipment was provided and maintained to minimize the risk of accidents or exposures. Additionally, continuous monitoring and assessment of the experimental conditions were carried out to promptly identify and address any potential safety hazards. This all-encompassing approach to providing guidance for experiment development and ensuring safety was crucial for the successful and reliable execution of the research project.

Tasks:

Public Engagement

Specific tasks:

Coach and assist team members in the following: Through glucose concentration gradient plate experiments, the compatibility of chassis bacteria EcN with the experiment was verified and evaluated. In order to simulate different blood glucose concentrations, L B. Agar plate: L. without glucose B. Agarose agar plate, L. containing 2.0 mmol/L glucose B. Agar plates, L. agar plates containing 5.0 mmol/L glucose, L-B agar plates containing 8.0 mmol/L glucose, and L-B agar plates containing 11.0 mmol/L glucose are used to represent the low, normal, and high blood glucose ranges. Subsequently, these agar plates were inoculated with the same dilution factor of chassis ECN1917 and engineering bacteria containing introduced plasmids to evaluate their growth status. The results showed that all types of bacteria grew normally on various plates, and there was no statistically significant difference in colony counts.

Tasks:

Software, Hardware, Wiki Coding, Visualization, Writing

Specific tasks:

(1) In order to stimulate the public's interest in medicine and improve medical students' understanding of anatomy and art, an online anatomy and art drawing activity was planned - online anatomy and art painting activity. Combining medical knowledge with artistic creation, this event aims to foster a strong interest in anatomy among medical students, while allowing the public to understand medical knowledge in a more acceptable way. (2) In order to become a disseminator of health knowledge, aiming to spread the light of science to every corner of the community, especially for the current growing problem of type 2 diabetes, we bring a vivid and practical health lecture to the residents of the Gaolan Road community. (3) Organize a series of community outreach activities, such as lectures, interactive experiences and questionnaires, to integrate the knowledge of type 2 diabetes prevention into the community and create a collective atmosphere for the prevention of type 2 diabetes in the whole society. (4) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (5) Organize scientific knowledge dissemination activities in primary and secondary schools in the underdeveloped northwest region to solve the problem of educational inequality and remove barriers to the dissemination of biological and health science knowledge. (6)Wrote a business plan.

Tasks:

Other, Entrepreneurship, Investigation, Background Research, Safety

Specific tasks:

Coach and assist team members in the following: Through glucose concentration gradient plate experiments, the compatibility of chassis bacteria EcN with the experiment was verified and evaluated. In order to simulate different blood glucose concentrations, L B. Agar plate: L. without glucose B. Agarose agar plate, L. containing 2.0 mmol/L glucose B. Agar plates, L. agar plates containing 5.0 mmol/L glucose, L-B agar plates containing 8.0 mmol/L glucose, and L-B agar plates containing 11.0 mmol/L glucose are used to represent the low, normal, and high blood glucose ranges. Subsequently, these agar plates were inoculated with the same dilution factor of chassis ECN1917 and engineering bacteria containing introduced plasmids to evaluate their growth status. The results showed that all types of bacteria grew normally on various plates, and there was no statistically significant difference in colony counts.

Tasks:

Other

Specific tasks:

Coach and assist team members in the following: (1) In order to stimulate the public's interest in medicine and improve medical students' understanding of anatomy and art, an online anatomy and art drawing activity was planned - online anatomy and art painting activity. Combining medical knowledge with artistic creation, this event aims to foster a strong interest in anatomy among medical students, while allowing the public to understand medical knowledge in a more acceptable way. (2) In order to become a disseminator of health knowledge, aiming to spread the light of science to every corner of the community, especially for the current growing problem of type 2 diabetes, we bring a vivid and practical health lecture to the residents of the Gaolan Road community. (3) Organize a series of community outreach activities, such as lectures, interactive experiences and questionnaires, to integrate the knowledge of type 2 diabetes prevention into the community and create a collective atmosphere for the prevention of type 2 diabetes in the whole society. (4) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (5) Organize scientific knowledge dissemination activities in primary and secondary schools in the underdeveloped northwest region to solve the problem of educational inequality and remove barriers to the dissemination of biological and health science knowledge

Tasks:

Other, Writing, Visualization, Public Engagement

Specific tasks:

(1) In order to stimulate the public's interest in medicine and improve medical students' understanding of anatomy and art, an online anatomy and art drawing activity was planned - online anatomy and art painting activity. Combining medical knowledge with artistic creation, this event aims to foster a strong interest in anatomy among medical students, while allowing the public to understand medical knowledge in a more acceptable way. (2) In order to become a disseminator of health knowledge, aiming to spread the light of science to every corner of the community, especially for the current growing problem of type 2 diabetes, we bring a vivid and practical health lecture to the residents of the Gaolan Road community. (3) Organize a series of community outreach activities, such as lectures, interactive experiences and questionnaires, to integrate the knowledge of type 2 diabetes prevention into the community and create a collective atmosphere for the prevention of type 2 diabetes in the whole society. (4) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (5) Organize scientific knowledge dissemination activities in primary and secondary schools in the underdeveloped northwest region to solve the problem of educational inequality and remove barriers to the dissemination of biological and health science knowledge.(6)Beautify the webpage and assist in carrying out related activities.

Tasks:

Other, Public Engagement

Specific tasks:

(1) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (2) Invite students from the main campus of Lanzhou University to visit our team's laboratory, so that they have the opportunity to experience the charm of biology up close. (3) Hold an event with the theme of "lzu-medicine-china creates a biomedical film journey". lanzhou university students were invited to visit our affiliated hospital, where they detailed the remarkable achievements and exciting projects that our lzu-medicine-china team has achieved over the years. Students visited the laboratory and performed simple experiments such as DNA extraction and agarose gel electrophoresis under the guidance of their supervisors. (4) In response to the call of the World Health Organization for popular science education, and to improve students' understanding of synthetic biology and type 2 diabetes, a popular science answer lottery was carefully planned. This event is not only a feast of knowledge, but also a journey of popular science. Through interactive Q&A, we aim to spark students' interest in cutting-edge science, especially in the emerging field of synthetic biology, and in the prevention and treatment of type 2 diabetes.(5)Beautify the webpage and assist in carrying out related activities.

Tasks:

Other, Writing, Software, Public Engagement, Wet Lab, Analysis, Investigation, Background Research

Specific tasks:

(1) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (2) Invite students from the main campus of Lanzhou University to visit our team's laboratory, so that they have the opportunity to experience the charm of biology up close. (3) Hold an event with the theme of "lzu-medicine-china creates a biomedical film journey". lanzhou university students were invited to visit our affiliated hospital, where they detailed the remarkable achievements and exciting projects that our lzu-medicine-china team has achieved over the years. Students visited the laboratory and performed simple experiments such as DNA extraction and agarose gel electrophoresis under the guidance of their supervisors. (4) In response to the call of the World Health Organization for popular science education, and to improve students' understanding of synthetic biology and type 2 diabetes, a popular science answer lottery was carefully planned. This event is not only a feast of knowledge, but also a journey of popular science. Through interactive Q&A, we aim to spark students' interest in cutting-edge science, especially in the emerging field of synthetic biology, and in the prevention and treatment of type 2 diabetes.(5)Beautify the webpage and assist in carrying out related activities.(6) Lab work: Through glucose concentration gradient plate experiments, the compatibility of chassis bacteria EcN with the experiment was verified and evaluated. In order to simulate different blood glucose concentrations, L B. Agar plate: L. without glucose B. Agarose agar plate, L. containing 2.0 mmol/L glucose B. Agar plates, L. agar plates containing 5.0 mmol/L glucose, L-B agar plates containing 8.0 mmol/L glucose, and L-B agar plates containing 11.0 mmol/L glucose are used to represent the low, normal, and high blood glucose ranges. Subsequently, these agar plates were inoculated with the same dilution factor of chassis ECN1917 and engineering bacteria containing introduced plasmids to evaluate their growth status.

Tasks:

Other, Fundraising

Specific tasks:

Help our team raise funds.Coach and assist team members in the following: (1) In order to stimulate the public's interest in medicine and improve medical students' understanding of anatomy and art, an online anatomy and art drawing activity was planned - online anatomy and art painting activity. Combining medical knowledge with artistic creation, this event aims to foster a strong interest in anatomy among medical students, while allowing the public to understand medical knowledge in a more acceptable way. (2) In order to become a disseminator of health knowledge, aiming to spread the light of science to every corner of the community, especially for the current growing problem of type 2 diabetes, we bring a vivid and practical health lecture to the residents of the Gaolan Road community. (3) Organize a series of community outreach activities, such as lectures, interactive experiences and questionnaires, to integrate the knowledge of type 2 diabetes prevention into the community and create a collective atmosphere for the prevention of type 2 diabetes in the whole society. (4) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (5) Organize scientific knowledge dissemination activities in primary and secondary schools in the underdeveloped northwest region to solve the problem of educational inequality and remove barriers to the dissemination of biological and health science knowledge

Tasks:

Writing, Wiki Coding, Hardware, Visualization, Software

Specific tasks:

(1) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (2) Invite students from the main campus of Lanzhou University to visit our team's laboratory, so that they have the opportunity to experience the charm of biology up close. (3) Hold an event with the theme of "lzu-medicine-china creates a biomedical film journey". lanzhou university students were invited to visit our affiliated hospital, where they detailed the remarkable achievements and exciting projects that our lzu-medicine-china team has achieved over the years. Students visited the laboratory and performed simple experiments such as DNA extraction and agarose gel electrophoresis under the guidance of their supervisors. (4) In response to the call of the World Health Organization for popular science education, and to improve students' understanding of synthetic biology and type 2 diabetes, a popular science answer lottery was carefully planned. This event is not only a feast of knowledge, but also a journey of popular science. Through interactive Q&A, we aim to spark students' interest in cutting-edge science, especially in the emerging field of synthetic biology, and in the prevention and treatment of type 2 diabetes. (5)Assist in creating Wiki.

Tasks:

Other, Writing, Wiki Coding

Specific tasks:

(1) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (2) Invite students from the main campus of Lanzhou University to visit our team's laboratory, so that they have the opportunity to experience the charm of biology up close. (3) Hold an event with the theme of "lzu-medicine-china creates a biomedical film journey". lanzhou university students were invited to visit our affiliated hospital, where they detailed the remarkable achievements and exciting projects that our lzu-medicine-china team has achieved over the years. Students visited the laboratory and performed simple experiments such as DNA extraction and agarose gel electrophoresis under the guidance of their supervisors. (4) In response to the call of the World Health Organization for popular science education, and to improve students' understanding of synthetic biology and type 2 diabetes, a popular science answer lottery was carefully planned. This event is not only a feast of knowledge, but also a journey of popular science. Through interactive Q&A, we aim to spark students' interest in cutting-edge science, especially in the emerging field of synthetic biology, and in the prevention and treatment of type 2 diabetes.(5)Participate in writing a wiki.

Tasks:

Conceptualization, Other, Safety

Specific tasks:

Provided guidance for the development of experiments and ensuring safety.Provided comprehensive and detailed guidance for the development of a series of experiments, taking into account various factors and potential challenges that may arise during the experimental process. This guidance not only included the scientific and technical aspects such as experimental design, methodology selection, and data analysis, but also emphasized the importance of ensuring safety throughout the entire experimental procedure. Safety measures were carefully formulated and implemented to protect the researchers, the experimental environment, and the integrity of the experiments. Regular safety training sessions were organized to familiarize the research team with safety protocols and emergency response procedures. Appropriate safety equipment was provided and maintained to minimize the risk of accidents or exposures. Additionally, continuous monitoring and assessment of the experimental conditions were carried out to promptly identify and address any potential safety hazards. This all-encompassing approach to providing guidance for experiment development and ensuring safety was crucial for the successful and reliable execution of the research project.

Tasks:

Other, Wiki Coding, Writing, Software

Specific tasks:

(1) In order to stimulate the public's interest in medicine and improve medical students' understanding of anatomy and art, an online anatomy and art drawing activity was planned - online anatomy and art painting activity. Combining medical knowledge with artistic creation, this event aims to foster a strong interest in anatomy among medical students, while allowing the public to understand medical knowledge in a more acceptable way. (2) In order to become a disseminator of health knowledge, aiming to spread the light of science to every corner of the community, especially for the current growing problem of type 2 diabetes, we bring a vivid and practical health lecture to the residents of the Gaolan Road community. (3) Organize a series of community outreach activities, such as lectures, interactive experiences and questionnaires, to integrate the knowledge of type 2 diabetes prevention into the community and create a collective atmosphere for the prevention of type 2 diabetes in the whole society. (4) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (5) Organize scientific knowledge dissemination activities in primary and secondary schools in the underdeveloped northwest region to solve the problem of educational inequality and remove barriers to the dissemination of biological and health science knowledge. (6)Participate in writing a wiki.

Tasks:

Other, Writing, Visualization, Wiki Coding

Specific tasks:

(1) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (2) Invite students from the main campus of Lanzhou University to visit our team's laboratory, so that they have the opportunity to experience the charm of biology up close. (3) Hold an event with the theme of "lzu-medicine-china creates a biomedical film journey". lanzhou university students were invited to visit our affiliated hospital, where they detailed the remarkable achievements and exciting projects that our lzu-medicine-china team has achieved over the years. Students visited the laboratory and performed simple experiments such as DNA extraction and agarose gel electrophoresis under the guidance of their supervisors. (4) In response to the call of the World Health Organization for popular science education, and to improve students' understanding of synthetic biology and type 2 diabetes, a popular science answer lottery was carefully planned. This event is not only a feast of knowledge, but also a journey of popular science. Through interactive Q&A, we aim to spark students' interest in cutting-edge science, especially in the emerging field of synthetic biology, and in the prevention and treatment of type 2 diabetes.(5)Participate in writing a wiki.

Tasks:

Other, Conceptualization, Analysis, Fundraising, Safety

Specific tasks:

Provide financial support and theoretical guidance for our project.Provided comprehensive and detailed guidance for the development of a series of experiments, taking into account various factors and potential challenges that may arise during the experimental process. This guidance not only included the scientific and technical aspects such as experimental design, methodology selection, and data analysis, but also emphasized the importance of ensuring safety throughout the entire experimental procedure. Safety measures were carefully formulated and implemented to protect the researchers, the experimental environment, and the integrity of the experiments. Regular safety training sessions were organized to familiarize the research team with safety protocols and emergency response procedures. Appropriate safety equipment was provided and maintained to minimize the risk of accidents or exposures. Additionally, continuous monitoring and assessment of the experimental conditions were carried out to promptly identify and address any potential safety hazards. This all-encompassing approach to providing guidance for experiment development and ensuring safety was crucial for the successful and reliable execution of the research project.

Tasks:

Other, Wiki Coding, Writing

Specific tasks:

(1) In order to stimulate the public's interest in medicine and improve medical students' understanding of anatomy and art, an online anatomy and art drawing activity was planned - online anatomy and art painting activity. Combining medical knowledge with artistic creation, this event aims to foster a strong interest in anatomy among medical students, while allowing the public to understand medical knowledge in a more acceptable way. (2) In order to become a disseminator of health knowledge, aiming to spread the light of science to every corner of the community, especially for the current growing problem of type 2 diabetes, we bring a vivid and practical health lecture to the residents of the Gaolan Road community. (3) Organize a series of community outreach activities, such as lectures, interactive experiences and questionnaires, to integrate the knowledge of type 2 diabetes prevention into the community and create a collective atmosphere for the prevention of type 2 diabetes in the whole society. (4) Conceptualization - Propose the plan and details of the project implementation; Visualization - Design posters, wiki pages, team logos, team mascots, team uniforms, edited images, etc. (5) Organize scientific knowledge dissemination activities in primary and secondary schools in the underdeveloped northwest region to solve the problem of educational inequality and remove barriers to the dissemination of biological and health science knowledge. (6)Participate in writing a wiki.