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Basic Parts

Part Number Name Type Brief Introductions
BBa_K5261000 TFD-EE Coding This is a double-stranded protein that adsorbs rare earth ions and luminesces through tryptophan.
BBa_K5261001 TFD-S Coding This is a single-chain protein that adsorbs rare earth ions and luminesces through tryptophan.
BBa_K5261002 TFD-QQ Coding This is a double-stranded protein that mutates the rare-earth metal binding site of TFD-EE so that it loses its rare-earth adsorption capacity.
BBa_K5261003 TFD-M Coding This is a single-chain protein that adds a set of rare-earth binding sites to the bottom of the TIM barrel based on TFD-S.
BBa_K5261004 TFD-R Coding This is a single-chain protein that mutates the original rare-earth binding site of TFD-S based on TFD-M so that it has rare-earth binding capacity only at the bottom of the TIM barrel.
BBa_K5261011 T7 tag Tag A label for screening.
BBa_K5261012 Xpress tag Tag A label for screening.
BBa_K5261013 V5 tag Tag A label for screening.
BBa_K5261015 FLO11 Coding The FLO11 gene encodes a serine-and threonine-rich GPI-anchored glycoprotein that enhances cell-to-cell and cell-to-substrate carrier interactions in the presence of Ca(Ⅱ) in solution.
BBa_K5261016 pTEF1 Regulatory pTEF1 is a strong promoter for overexpression of the FLO11 gene.
BBa_K5261020 pCTR3 Regulatory The CTR3 promoter is a repressive promoter in response to copper ions and is usually repressed at higher copper concentrations.


Composite Parts

Part Number Name Type Brief Introductions
BBa_K5261010 AGA2-GS linker-T7 tag-Xpress tag-TFD S-V5 tag-6xHis composite Yeast surface display pathway
BBa_K5261017 pTEF1-FLO11 composite Bioadhesion expression pathway
BBa_K5261021 pCTR3-mCherry-tCYC1 composite CTR3 promoter strength characterisation pathway