Project Description

Describe how and why you chose your iGEM project.

Bronze Medal Criterion #3

Describe how and why you chose your iGEM project.


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Study on the efficiency and mechanism of photosynthetic bacteria in treating high salinity organic wastewater for protein production


With the rapid development of petrochemical, pharmaceutical, electric power, dyestuff, tannery, food processing and other industries, the discharge of high salinity organic wastewater is increasing. High salinity organic wastewater is not only rich in organic pollutants, but also contains a large number of soluble inorganic salts. The direct discharge of high salinity organic wastewater will cause great pollution to the environment. Biological methods are considered as a potential method due to their cost-effectiveness, environmental friendliness and high organic pollutant removal rate. Currently, some conventional biological methods, such as activated sludge and membrane bioreactors, have been applied to the treatment of high-salinity organic wastewater. However, these biological methods have the disadvantages of low efficiency and complicated operation in treating high salinity organic wastewater. Photosynthetic bacteria (PSB) are a group of prokaryotic organisms with a primitive light-energy synthesizing system. PSB have been widely used in wastewater treatment, aquaculture, plantations, animal husbandry, and new energy development due to their diversity of types, metabolic modes, and metabolites. PSB wastewater treatment, as an emerging technology, not only achieves the removal of organic matter, but also synthesizes, through its metabolism, coenzyme Q10 (CoQ10), carotenoids, proteins, and 5-aminolevulinic acid (5-ALA) through its metabolism, thus realizing efficient wastewater treatment and regeneration of material resources. Therefore, PSB is promising for the treatment of high-salt organic wastewater and resource utilization. The aim of this project is to screen and construct salt-tolerant PSBs to realize efficient treatment of high-salt organic wastewater treatment to generate microbial proteins. The specific content includes:
(1) the targeted domestication of salt-tolerant PSB strains;
(2) the analysis and regulation of PSB salt-tolerance mechanism;
(3) the construction and optimization of the system for the efficient treatment of high-salt organic wastewater by PSB to produce microbial proteins.
This project is of great significance for the production of high value-added products by PSB treatment of high-salinity organic wastewater, which can grow the treatment of high-salinity organic wastewater and provide technical guarantee to support the construction of ecological civilization.

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References


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