Safety

Safety is not merely the priority; it is the pillar upon which every sensible laboratory project relies and flourishes. In our iGEM project, we have the conviction that a safe lab environment encourages ingenuity, teamwork, and the pursuit of excellence. By weaving safety into the very core of our mission, we make it clear that our research is not only about expanding our minds but also about protecting the health of our team and the welfare of the whole community. This section of our document defines the ways we manage safety issues, systems integrated into the design of the project, and procedures to be followed to establish an environment of research that is safe and responsible.

We have selected using non-pathogenic forms of Escherichia coli(BL21, Rosetta, lysY and OrigamiB), as designated chassis organisms. These organisms are extensively characterized and renowned for their easy use in research, and they are also seen as having little hazard to humans, other animals, and the environment in general.

All parts used in our project are selected to ensure they do not pose harm to humans, animals, or plants. We avoid using parts that could be hazardous on their own or in our project.

Where possible, we have substituted safer materials for more hazardous ones. For example, we carefully handle and minimize the use of hazardous chemicals like TEMED, polyacrylamide, and methanol, ensuring proper safety protocols are in place.

we are exploring the inclusion of an "induced lethality" or "kill-switch" device to prevent any accidental release of engineered organisms into the environment. This mechanism would ensure that our engineered bacteria can be safely and effectively terminated if necessary.

Daily Safety Procedures

All team members wear lab coats, gloves, and eye protection at all times in the lab. We use a chemical fume hood when handling volatile or hazardous chemicals to minimize exposure to fumes. Team members receive comprehensive training on laboratory safety techniques, including handling chemicals and biological samples. New members practice under supervision to ensure they are competent and confident in performing experiments safely.

Unusual Experiments and Safety Issues

During PAGE gel preparation, we take extra precautions to avoid exposure to TEMED, acrylamide, and ammonium persulfate. This includes using appropriate PPE and working within the fume hood. During Coomassie Blue staining, we handle acetic acid with care, ensuring proper ventilation and PPE to prevent exposure through fumes or skin contact. During Western blotting buffer preparation, we handle anhydrous methanol with caution, using the fume hood and PPE to mitigate risks.

Documentation and Reporting

We maintain a strict documentation system, recording all laboratory activities. This ensures traceability for all experiments conducted. Any accidents or unusual incidents are promptly reported and recorded in our accident reporting system, ensuring that corrective actions can be taken and future risks minimized.

Risk Management Actions

Through comprehensive safety training and supervised practice, team members are trained to perform experiments calmly and consciously. We use a chemical fume hood when handling any volatile chemicals. We enforce a strict PPE policy, ensuring all team members wear gloves and appropriate protective gear in the laboratory. We implement a strict partner and supervision system, as well as a documentation system, to ensure safety and traceability in all laboratory activities.

Collaboration with Experts

We consult regularly with institutional laboratory safety officers and experienced biology teachers to manage risks effectively. Our safety protocols align with national regulations, including the Biological Security Law of the People's Republic of China and the Chinese Educational Laboratory Safety Regulations.