Determining E. coli Nissle 1917 as a compatible probiotic for our project.
Determining experimental methods for stomach acid carriers.
Consulting with professors regarding probiotic sources.
Integrating project information.
Dry Lab:
Excluding the stress relief lamp as hardware application.
Deciding to use beverages as the starting point for hardware development.
Dividing the design of the film into two directions; a layer of film attached under the sealing film of the daily beverage and a film hanging on the wall of the beverage cup.
Confirming the zinc finger binding domain sequences and preparing for protein modeling.
Human Practices:
Investigating the evidence that serotonin supplementation can rapidly enhance mood.
Gathering and analyzing global data on the prevalence of depression.
Wiki:
Conducting color research for the Wiki.
Selecting a Wiki style that aligns with our topic.
Week 2. (May 6th-12th)
Wet Lab:
Exploring the regulatory methods of 5-HTP production.
Exploring the oxygen-induced suicide mechanism for the biosafety strategy.
Planning the open lab summer camp.
Dry Lab:
Discussing how to present hardware and software in oral presentation.
Exploring materials that are more suitable for edible and water-soluble film.
Human Practices:
Mining the causes of negative moods and the effects of prolonged negative emotions.
Planning and brainstorming activities to enhance engagement and outreach to a broader audience.
Week 3. (May 13th-19th)
Wet Lab:
Integrating the information of two plasmids for 5-HTP production and enhancement.
Designing open lab summer camp content.
Determining the role of protocatechuic acid as a regulator of our plasmid.
Writing the safety form.
Dry Lab:
Choosing duck feet gelatin, fish skin, maltodextrin and starch as candidates of edible film.
The plasticizers that can be used for starch films are glycerin, sorbitol, and polyethylene glycol.
Deciding to simulate the concentration and calculate the production of 5-HTP to obtain the amount of bacterial powder required for the purpose of mathematical modeling.
Completing the calculation of 5-HTP production as intracellular tryptophan concentration increases.
Completing the language conversion, basic user interface, Fourier transformation, and data export functions for APP.
Human Practices:
Analyzing the specific objectives of each planned activity.
Determining themes for upcoming social media posts.
Week 4. (May 20th-26th)
Wet Lab:
Integrating information for the open lab summer camp.
Determining the regulation of 5-HTP production.
Writing the safety form.
Dry Lab:
Confirming the film will be attached beneath the sealing film of daily beverage.
Searching for the material and protocol of film.
Mathematical modeling of 5-HTP production after increasing to three enzymes.
Simulating mutTPH1 and finding out its similar sequence protein: 3HF6.
Showing the stress level on software.
Finding professors for suggestions for software improvement.
Supporting Open Lab on the introduction of 3D printers.
Human Practices:
Organizing workshops to enhance the public's understanding of emotions.
Posting registration information for Open Lab sessions on Instagram.
Wiki:
Deciding and planning Wiki homepage design.
Week 5. (May 27th-2nd)
Wet Lab:
Analyzing the pros and cons of 5-HTP production from probiotics.
Analyzing and formulating the biosafety strategy.
Dry Lab:
Confirming the protocol of maltodextrin and starch film.
Searching for the information of the 3D bioprinter to find out whether it can be used in hardware.
Optimizing the simulation of tryptophan absorption and in vivo reactant concentrations.
Confirming the necessary constant in mathematical modeling.
Using BH4 data to simulate the relationship between MH4 concentration and reaction rate (TPH_Vmax).
Simulating the fusion protein of TPH1 + zif268.
Simulating DHPR and finding out its similar sequence protein: 1DHRA.
Simulating PCD and finding out its similar sequence proteins: 1F93.
Adding the results of the pressure test and the notification that will pop up when the pressure is too high for software.
Human Practices:
Exploring the frequency of hand-shaken drink consumption among Taiwanese individuals and identifying the demographics involved.
Brainstorming ways to make foreigners understand about hand-shaken drinks.
Week 1. (Jun. 3rd-9th)
Wet Lab:
Analyzing the differences between 5-HTP production from probiotics and supplements.
Determining some proteins for biosafety.
Designing a vector map in SnapGene.
Dry Lab:
Writing a project description of our film.
Organizing questions for a software interview.
Human Practices:
Focusing on relationships, stress, and emotions.
Deciding to organize separate activities for younger and older people.
Planning to organize activities for the local community, focusing on emotional care for the elderly.
Wiki:
Designing a detailed wiki page, especially for the “Project Description” page.
Assigning color codes to each element on the wiki.
Week 2. (Jun. 10th-16th)
Wet Lab:
Analyzing experimental method to replace the ampicillin resistance gene with a kanamycin resistance gene in pST39.
Designing the open lab summer camp experimental course.
Dry Lab:
Writing a project description for edible film.
Optimizing questions for the software interview.
Human Practices:
Arranging the schedule for activities and assigning responsibilities.
Reconsidering the Free Hug campaign and simultaneously initiating a Hug Marathon event to promote our concept.
Wiki:
Designing the loading page for the wiki.
Determining the font styles for each section.
Coding the menu and footer of wiki.
Week 3. (Jun. 17th-23rd)
Wet Lab:
Replacing the ampicillin resistance gene in the pST39 vector with a kanamycin resistance gene.
Designing the presentation and textbook for the open lab summer camp.
Demonstrating the open lab summer camp experimental course.
Dry Lab:
Listing out future progress schedules.
Confirming the protocols of different kinds of film.
Adding soy protein and sodium caseinate film as edible film candidates.
Adding peptide linker for fusion protein modeling.
Finishing simulating the fusion proteins with linkers: TPH1 + zif268, PCD + PSBll.
Human Practices:
Deciding to conduct a survey due to the uncertainty of the workshop.
Establishing the details of activity of Free Hug campaign.
Scheduling the workshops.
Inquiring about the timing, preparing the proposal, and approaching the manager of the community center regarding the Elderly Seniors Program.
Proposing street surveys and interviewing local residents.
Reviewing the objectives that the events need to achieve and providing an overview of the event.
Wiki:
Typesetting the page of the project description.
Week 4. (Jun. 24th-30th)
Wet Lab:
Replacing the ampicillin resistance gene in the pST39 vector with a kanamycin resistance gene.
Completing the presentation and textbook for the open lab summer camp.
Printing out the textbook for the open lab summer camp.
Dry Lab
Searching the guideline of the film.
Determining the dissolving rate, liquid viscosity and transparency as the selection criteria of edible film.
Calculating the production of 5-HTP.
Simulating the fusion protein of DHPR + ZFa.
Finishing the powerpoint of the introduction of 3D printers for the Open Lab.
Human Practices:
Canceling the workshop and transitioning to an "Emotional Awareness" lecture.
Confirming the speaker for the lecture, as well as the time, location, and anticipated number of attendees.
Determining the format of the questionnaire for the street survey.
Planning to organize an online lecture.
Preparing the survey for local chain beverage stores.
Wiki:
Putting the content onto the page of the project description.
Week 1. (Jul. 1st-7th)
Wet Lab:
Previewing the open lab course.
Replacing Ampicillin resistance gene in plasmids with Kanamycin resistance gene.
Dry Lab:
Performing the preliminary experiment of film.
Searching for the protocol to examine viscosity of film.
Optimizing experiments of dissolving rate, viscosity and transparency of the film.
Modeling the production rate of 5-HTP.
Preparing the interview with professors to improve protein modeling.
Concluding results of modeling contents on wiki.
Adding the function to alert users in the app when the users measured pressure exceeds the limit for the software.
Adding the phone notification permission for the software.
Human Practices:
Confirming team-building benefits and discounts, discussing the cost and quantity of cup sleeves, and finalizing team uniforms by July 15.
Finalizing the survey content, focusing on recent events.
Designing a poster for the international student free hug campaign, with an emphasis on cultural exchange.
Planning an emotional care event for August 30, considering the involvement of potential psychological experts. Preparing content for store managers and designing an iGEM banner.
Preparing to visit nearby beverage stores to promote our project and conduct surveys from the store;s perspective.
Planning an emotional care event on August 30, considering potential psychological experts to involve. Preparing content for store managers and designing an iGEM banner.
Preparing to visit beverage stores nearby to promote our project and conducting surveys from the store's perspective.
Scheduling online lectures and finding speakers..
Designing team logo.
Week 2. (Jul. 8th-14th)
Wet Lab:
Attending the experimental course.
Replacing Ampicillin resistance gene in plasmids with Kanamycin resistance gene.
Dry Lab:
Finalizing the protocol of the dissolution rate experiment.
Revising the experimental design for viscosity.
Estimating the costs of different film types.
Interviewing professors to improve software.
Debugging based on the interview content for software.
Finishing the design of the software icon.
Adding the schedule planning function for the software.
Redesigning the user interface for the software.
Human Practices:
Finalizing the team logo and holding a vote.
Replacing online lectures with an interview featuring a psychology professor.
Visiting beverage stores nearby to promote our project and conducting surveys from the store's perspective.
Revising the survey content and order, then posting it on social media after receiving approval from a supervisor.
Coordinating the event schedule with store managers and discussing plans with a physical therapist, including banner creation and logistics.
Wiki:
Discussing Wiki design details and positioning.
Gathering information on team members for the 'Members' section.
Revising minor details of the Wiki.
Week 3. (Jul. 15th-21st)
Wet Lab:
Ligating TPH1 and QDPR inserts into the plasmid pST39 by Gibson assembly.
Transforming pST39-TPH1 and pST39-QDPR into DH5a for selection.
Completing the first batch of the open lab experimental course.
Dry Lab:
Confirming the experimental methods and materials for the film and conducting the experiment.
Finishing the first batch of dissolution rate experiments for film by using resistance measurements.
Designing and developing a hardware device to measure the dissolution test.
Using Spectrophotometry to test the dissolution rate for films of different materials.
Testing the new software: VMD for protein modeling.
Adding the function to remind users to practice diaphragmatic breathing for relaxation for software.
Taking the frequency method of HRV out of the measurement.
Human Practices:
Preparing to film a promotion video and discussing the storyline to ensure a compelling promotional video.
Scheduling an appointment for the expert interview, finalizing details with the psychology professor.
Wiki:
Designing the 'Team Members' Wiki page.
Adding the loader for Wiki.
Trying the flip cards of 'Team Members'.
Fixing the position of the sidebar.
Week 4. (Jul. 22nd-28th)
Wet Lab:
Sequencing plasmids pST39-TPH1 and pST39-QDPR.
Completing the second batch of the open lab experimental course.
Dry Lab:
Organizing the data for the first batch of dissolution rate experiments for film.
Completing the second run of dissolution rate experiments by spectrophotometer.
Deciding to exclude the experiment for the viscosity of film.
Testing the alphaphold2 for protein modeling with the help of an adviser.
Changing the measurement time to 3 minutes for the software.
Keeping the last 10 seconds and deleting the first 10 seconds in data pre-processing for the software.
Detailed planning of the progress for each item.
Human Practices:
Compiling and reviewing the results from the beverage store visits to analyze the feedback and insights gathered.
Planning to visit the Culture night market with posters, QR codes, and a lucky draw activity to engage with participants.
Wiki:
Designing the notebook and sponsorships page.
Setting deadlines for notebook content.
Adding a progress bar for every page.
Week 5. (Jul. 29th-Aug. 4th)
Wet Lab:
Culturing bacteria for microencapsulation experiments.
Inserting the protocatechuic acid (PCA) regulatory cassette into the vector.
Dry Lab:
Preparing interviews for film.
Designing humidity experiment for film.
Referring to the result from iTASSER, use AlphaFold2 to run again the protein modeling.
Composing the menu and work and rest planning in the app.
Human Practices:
Deciding on the next visit to the night market and adjusting the activities accordingly.
Heading to the Culture Night Market for a free hug event, distributing questionnaires, and conducting a quick emotional state survey.
Arranging team photoshoot session.
Wiki:
Coding 'Team Member' page.
Week 1. (Aug. 5th-11th)
Wet Lab:
Checking the expression of the following proteins: pST39 - TPH1, pST39 - PCBD1, pST39 - QDPR.
Sequencing the plasmid pST39-PCA.
Preparing materials for the microencapsulation experiment.
Dry Lab:
Organizing the result of soy protein, sodium caseinate and starch film.
Contacting the film sealing machine manufacturer for an interview.
Designing the liquid viscosity test.
Repairing the bar chart of the app.
Designing the posters.
Human Practices:
Organizing a 'Free Hug' event for international students at the university.
Seeking fundraising support from several temples in Chiayi.
Posting updates on social media to introduce our iGEM team and project.
Developing questions for interviewing the experts.
Wiki:
Coding 'Notebook' page.
Adding May and June notebook into 'Notebook' page.
Week 2. (Aug. 12th-18th)
Wet Lab:
Checking the expression of the following proteins: pST39 - TPH1 + PCBD1, pST39 - PCBD1 + QDPR, pST39 - QDPR + TPH1.
Inserting the KanR cassette into the vector.
Conducting a microencapsulation experiment.
Dry Lab:
Organizing the result of sodium caseinate film liquid viscosity test.
Formulating the preservation conditions for the film.
Coding two mathematical models with scaffold and no scaffold.
Designing the mathematical models of how PCA concentration affects gene expression.
Human Practices:
Collaborating online with the CSMU x NCHU iGEM team.
Collaborating online with the NYCU Formosa iGEM team.
Contacting and reaching out to international iGEM teams for collaborations.
Recording the second batch of the promotion video.
Posting the results of the 'Open Lab' event, including pictures and videos, on social media.
Wiki:
Designing Wiki homepage.
Retype setting 'Members' page.
Week 3. (Aug. 19th-25th)
Wet Lab:
Sequencing the plasmid pST39 - TPH1 + PCBD1 + QDPR.
Inserting the KanR cassette into the vector.
Analyzing the microencapsulation experiment using a Zetasizer.
Dry Lab:
Designing the humidity test for film.
Brainstorming the outline of 'Application'.
Modifying the outline of protein models.
Including the app background.
Including the feature where the water wave moves according to the waveform during measurement.
Human Practices:
Editing the second batch of promotional video.
Participating in the Taiwan iGEM Seminar for two days.
Preparing the questionnaire for the Emotion Awareness Seminar.
Designing and ordering the team banner.
Scheduling and brainstorming social media contents through to completion.
Wiki:
Rescheduling wiki content deadline.
Week 4. (Aug. 26th-Sep. 1st)
Wet Lab:
Checking the expression of the following proteins: pST39 - TPH1 + PCBD1 + QDPR.
Inserting the RCR cassette into the vector.
Preparing additional samples from the microencapsulation experiment for pH testing.
Dry Lab:
Designing an experiment to observe microbial growth at different humidity levels over a period of time.
Discussing future applications for film.
Managing the app beta version.
Human Practices:
Seeking fundraising support from Management College.
Organizing a physical exercise activity for the elderly at a social center.
Collaborating online with the HKUST iGEM team.
Finalizing the promotion video.
Finalizing 'Human Practices' Wiki outline.
Wiki:
Finalizing the Wiki homepage design.
Gathering information for attribution form.
Confirming Wiki all pages outline.
Week 1. (Sep. 2nd-8th)
Wet Lab:
Finalizing the analysis of experimental results for the induction of three fusion proteins.
Conducting gel extraction, colony PCR, and restriction digestion to verify plasmid size.
Testing encapsulated E. coli at different pH levels.
Writing Wiki content outline.
Dry Lab:
Deciding to exclude the experiment on bacterial growth in different humidity conditions.
Composing a reminder for the user to check their HRV in the app at 3 p.m. daily.
Finishing the meditation page and integrating the music page into the app.
Releasing a software demo and download tutorial.
Human Practices:
Completing and submitting the promotion video.
Promoting the open registration for the Emotion Awareness Seminar.
Introducing the iGEM competition and our current project through a campus campaign.
Starting to write Wiki content.
Wiki:
Gathering information for each page.
Typesetting 'Member' pages.
Week 2. (Sep. 9th-15th)
Wet Lab:
Testing the strength of the PCA promoter region with green fluorescent protein.
Recording the growth behavior of uncoated and coated E. coli.
Starting to write the Wiki content.
Dry Lab:
Conducting the Wiki content of the software.
Changing the suggestion for releasing stress will only appear after closing the window, which indicates high or severe stress levels in the software.
Changing the daily reminder time to 2 p.m. and adding the sound effect.
Addressing the issue of large file size for the software.
Human Practices:
Organizing a recruitment drive to enlist new members for the next iGEM team.
Holding the Emotion Awareness Seminar to help more people identify their own emotions.
Wiki:
Coding 'Home' page.
Week 3. (Sep. 16th-22nd)
Wet Lab:
Testing the strength of the PCA promoter region with green fluorescent protein.
Recording the growth behavior of coated E. coli.
Finishing the first version of Wiki content.
Dry Lab:
Using the precision instrument OMRON Intellisense HEM-7071 to verify the accuracy of the software measurement data.
Finishing the wiki content of protein modeling, application, and software.
Human Practices:
Finishing the first version of Wiki content.
Finishing the presentation video script.
Wiki:
Collecting pictures and information from each group.
