Synthetic biology empowers us with the ability to assemble complex biological systems much like assembling building blocks, with biological parts at the heart of this assembly process. Against this backdrop, we offer a glimpse into the diverse array of components utilized in the MuSecure project.
Among these components, you will find foundational elements that serve as the cornerstone of our work. For instance, we employ some basic parts, including MOR215 (BBa_K5187000), Gα (BBa_K5187001), pFUS1 promoter (BBa_K5187002), and ldhA (BBa_K5187003), to construct the core design of MuSecure.
Furthermore, we would like to highlight our impressive Muscone-gated molecular switch in Saccharomyces cerevisiae (BBa_K5187006), which was pioneered and characterized by Tsinghua University in 2024. This system forms the core of our project and holds great potential for widespread applications in the field of synthetic biology.
Our components have been designed to integrate muscone induction and the therapeutic capabilities of lactate. These parts embody our collective effort and wisdom, enabling us to achieve success in the laboratory work of our project.
| Part name | Part type | Short description |
|---|---|---|
| BBa_K5187000 | Protein Domain | MOR215-1: Receptor of Muscone |
| BBa_K5187001 | Protein Domain | GPA1-GNAI3: Synthetic Gα protein that responds to MOR215-1 |
| BBa_K5187002 | Regulatory | pFUS1: Downstream promoter for our artificial signaling pathway |
| BBa_K5187003 | Protein Domain | ldhA: lactate dehydrogenase |
| BBa_K5187004 | Protein Domain | ALS3: an adhesion protein |
| BBa_K5187005 | Regulatory | GAL1, 10 promoter: Bidirectional promoter |
| Part name | Part type | Short description |
|---|---|---|
| BBa_K5187006 | Composite | Muscone-gated molecular switch in Saccharomyces cerevisiae |
| BBa_K5187009 | Composite | Tetrathionate Sensing Colonization System |
Our aspiration extends beyond our immediate project goals. We envision these meticulously designed parts not only playing a pivotal role in advancing our project but also making significant contributions to the broader iGEM community and the ongoing development of synthetic biology as a comprehensive discipline.