Parts
Utilized and Characterized Existing Parts
| Gene Name | Part name | Description | Utilize and Characteration |
|---|---|---|---|
| LuxR |
BBa_C0062 |
In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr BBa_R0062, and repressing transcription from Pl BBa_R0063 |
Characterized the impact of different concentrations of
small molecules on the binding of LuxR to pLux. Through this mechanism, the characterization of real milk samples was achieved. |
| pLux |
BBa_R0062 |
The lux cassette of V. fischeri contains a left and a
right promoter. The right promoter gives weak
constitutive expression of downstream genes.This
expression is up-regulated by the action of the LuxR
activator protein complexed with the autoinducer,
3-oxo-hexanoyl-HSL. Two molecules of LuxR protein form a
complex with two molecules of the signalling compound
homoserine lactone (HSL). This complex binds to a
palindromic site on the promoter, increasing the rate of
transcription. This Plux promoter "pointing to the right" is the same sequence, but inverted, as part BBa_K199052 |
|
| P1 | BBa_J23101 |
iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P2 | BBa_J23102 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P3 | BBa_J23103 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P4 | BBa_J23104 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P5 | BBa_J23105 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P6 | BBa_J23107 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P7 | BBa_J23109 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P8 | BBa_J23110 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P9 | BBa_J23111 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P10 | BBa_J23112 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P11 | BBa_J23113 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P12 | BBa_J23114 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P13 | BBa_J23118 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P14 | BBa_K088007 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P15 | BBa_K137030 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
| P16 | BBa_R0085 | iGEM-contributed promoter |
Characterized the signal expression of LuxR under the control of this promoter |
New Composite Parts
| Description | Part name | Function |
|---|---|---|
| J23119-BBa_B0034-LuxR-BBa_B1006 | BBa_K5080000 | LuxR and pLux form a well-established transcriptional regulatory protein and its corresponding promoter. In bacteria, once LuxR binds to AHL, the resulting complex binds to the pLux promoter region, initiating the expression of downstream genes. The concentration of AHL is related to bacterial population density, and when the concentration exceeds a certain threshold, the LuxR-AHL complex activates the expression of downstream genes. This plasmid uses the strong promoter J23119 to drive high levels of LuxR protein expression in Escherichia coli, allowing it to work with BBa_K5080001 to detect the concentration of AHL molecules in the environment. |
| pLux-BBa_B0030-mVenusNB-BBa_B1005 | 🏆BBa_K5080001 |
pLux is a transcriptional regulator controlled by the
LuxR protein from Vibrio fischeri, responsible for
activating the expression of the luxICDABE gene cluster.
This gene cluster plays a critical role in bacterial
quorum sensing by detecting acyl-homoserine lactones
(AHLs) in the environment and triggering the synthesis
of bioluminescent proteins. By replacing the luxICDABE gene cluster with a fluorescent protein gene, a new biosensor was constructed that eliminates interference from the bacteria’s own production of AHLs, allowing it to specifically respond to AHL concentrations in the external environment. This design enables the sensor to more accurately detect and respond to AHL signal molecules in the environment. We further tested the combined use of this part with BBa_K5080000. The results showed that the biosensor accurately responded to different concentrations of AHLs in the environment and successfully detected the presence of AHLs in real milk samples. mVenusNB, also known as SYFP2, is a constitutively fluorescent yellow protein published in 2006, derived from the jellyfish Aequorea victoria. It is reported to be a very rapidly maturing monomeric fluorescent protein with moderate acid sensitivity. It is also an iGEM part: BBa_K864100. |
Medals and Awards