Parts
#Our team designed a template DNA called pRSFDuet-1_PdxA_PdxJ. We designed this template DNA to allow it to be used as a template sequence for the expression of PdxA_PdxJ proteins in a cell-free expression system. The following two tables provide information about the individual components of the template DNA pRSFDuet-1_PdxA_PdxJ and the information after the group meeting.
| Number | Name | Description |
|---|
| BBa_K5418000 | pRSFDuet-1 | As a vector. |
| BBa_K5418001 | PdxA | Gene sequence of rate-limiting enzyme in PN synthesis |
| BBa_K5418002 | PdxJ | Gene sequence of rate-limiting enzyme in PN synthesis |
| BBa_K5418003 | T7 Promoter | A promoter is a piece of DNA sequence that RNA polymerase recognizes, binds, and begins transcription. |
| BBa_K5418004 | RSP ori | It controls the replication of the plasmid and determines the host properties and copy number of the plasmid. |
| BBa_K5418005 | Kanr | This gene provides resistance to kanamycin and can be used to screen specific strains. |
| BBa_K5418007 | lacI | The lac repressor binds to the lac operator to inhibit transcription in E.coli. This inhibition can be relieved by adding lactose or isopropyl-β-D-thiogalactopyranoside(IPTG). |
| BBa_K5418008 | GFP | Reporter gene. |
Composite parts
#| Number | Name | Description |
|---|
| BBa_K5418009 | pRSFDuet-1_PdxA_PdxJ | As template DNA for CFPS. |
| BBa_K5418010 | pRSFDuet-1_PdxA_PdxJ-GFP | It is used to detect the expression of genes. |
