The bio-friendly production of vitamin B6 based on the cell-free expression system

1. The importance of VB6

- Prevention and Treatment of Disease

Vitamin B6 is a component of some coenzymes in the human body and is involved in a variety of metabolic reactions, especially closely related to amino acid metabolism. Vitamin B6 preparation is used in the prevention and treatment of vomiting during pregnancy and radiation sickness.

2. Hazards of Chemical Synthesis

- Environmental Pollution and Financial Burden

PN hydrochloride is the most common commercial form of vitamin B6 and is currently produced by complete chemical synthesis. At present, the mainstream method of PN synthesis is oxazole method, and the current research also focuses on the improvement of oxazole synthesis process. Although this method has the characteristics of easy availability of raw materials, high yield and low cost, the intermediates produced by the synthesis process are biotoxic and corrosive, which have a great impact on the environment and human body.

3. Limitations of intracellular synthesis

-The need of decoupling method

Vitamin B6 biosynthesis can be divided into de novo synthesis pathway and rescue pathway. However, both the de novo synthesis pathway and the salvage pathway are closely related to cell growth. Adjustments to both pathways would affect cell viability, forcing researchers to decouple the two pathways. This greatly increases the time cost and economic cost of biosynthesis.

4. VB6 Produced by CFPS

- Eco-Friendly, Economical and simple

In order to solve the above problems, we proposed to synthesize vitamin B6 using a cell-free expression system, which not only solved the pollution problem caused by chemical synthesis, but also solved the limitation of cell activity of biological synthesis.