Parts Collection

Introduction

The need for point-of-care sepsis diagnostics is critical, as early detection and intervention are essential for improving patient outcomes and reducing mortality. Traditional diagnostics often rely on centralized laboratories and lengthy processes, delaying crucial treatment decisions. DNA computing, when interfaced with CasX, offers a powerful solution by enabling rapid, programmable detection of pathogens and antimicrobial resistance directly at the point of care. This approach combines the precision of CRISPR technology with the flexibility of DNA-based logic gates, making it ideal for fast, accurate diagnostics. The modular design of our parts collection is essential to meet the growing demand for customizable, reprogrammable diagnostics that can be easily adapted to various pathogens, including resistant bacteria and viruses. This system empowers healthcare providers with the tools needed for timely, effective sepsis management.

Our synthetic biology parts collection is built on the principle of modularity, allowing for easy reengineering using a Python script and a dry lab DNA computing compiler tool. It includes reengineered tracrRNAs, CasX enzyme sequences, engineered dsDNA target strands for CasX, analog-to-digital converter components, helper and docker strands, and reporter gates—independently designed by McGill iGEM. Additionally, positive controls and measurement materials are included for calibration tests of these diagnostic devices.

See the Parts Registry pages for documentation and deeper explanations of components. For more explanation of the modularity of our system and re-engineering, see our Modularity page.