Welcome to our WiKi
Our project focuses on Enzymatic DNA
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Our team consists of 15 students and 3 mentors. Our students come from Nanjing, Beijing, Xi'an and other places around the world, and some of them are still attending high school abroad. In this iGEM event, our students overcame various difficulties and actively completed the tasks specified by iGEM.
Learn more about team members. Learn more about everyone's attributions.
Our project focuses on developing a new DNA synthesis technology using the enzymatic method.
This involves Terminal deoxynucleotidyl Transferase, or TdT, an unique DNA polymerase discovered in calf thymus. TdT becomes the most outstanding one because it doesn't require a template to synthesize DNA, making it ideal for the next-generation DNA synthesis.
Learn more about project description. Learn more about engineering. Learn more about model.
We successfully constructed and expressed ZaTdT from Zonotrichia albicollis. We also tested various sources of TdT, including Bovine Terminal Deoxynucleotidyl Transferase (Part: BBa_K1354002), for recombinant expression activity in an E. coli expression system.
We have collaborated with the Postgraduates Voluntary Teaching Corps of Nanjing University and Lecture Team of School of Life Sciences to leverage university resources in order to disseminate the concepts and knowledge of synthetic biology to a wider and more diverse audience.
Learn more about parts. Learn more about contribution.
In Human Practices section, our team did the following tasks:
Learn more about human practices.
Our members used the weekends and summer vacation to conduct experiments in the laboratory of Nanjing University School of Life Sciences. During the experiment, the three supervisors led our team members to conduct practical experiments, obtained the results of the experiment and conducted data analysis. This is the first time that most members have entered the biological laboratory for experimental operations. Everyone has harvested a lot. And at the same time, we have also gained precious friendship.
Learn more about safety. Learn more about experiments.