Brevibacillus borstelensis AK1, a thermophilic bacterium isolated from a hot spring in Saudi Arabia was selected for its potential to produce antimicrobial peptides. Bioinformatics analysis of its whole-genome sequencing identified several genes encoding potential antimicrobial peptides (AMPs). Six of these genes were chosen for testing based on their hit scores. All parts listed below was engineered by team KFUPM-Saudi-Arabia using components from the distribution kits plus the antimicrobial peptide genes found in our bacteria.
| Part Number | Part Origin | Type | Description | Designers | Length | Status |
|---|---|---|---|---|---|---|
| BBa_K5519001 | Brevibacillus borstelensis Ak1 APBN01000001.1 | Coding AMP | BborAMP1 is a novel antimicrobial peptide gene found in the genome sequence of the thermophilic bacteria Brevibacillus borstelensis AK1. This AMP is known for its ability to inhibit Staphylococcus aureus. | Latifat Abdulsalam | 117bp | In testing |
| BBa_K5519002 | Brevibacillus borstelensis Ak1 APBN01000001.1 | Coding AMP | BborAMP2 is a novel antimicrobial peptide gene found in the genome sequence of the thermophilic bacteria Brevibacillus borstelensis AK1. This AMP is known for its ability to inhibit Staphylococcus aureus. Our project aims at expressing this peptide in Escherichia coli, purifying and testing its ability to inhibit Staphylococcus aureus. | Latifat Abdulsalam | 69bp | In testing |
| BBa_K5519003 | Brevibacillus borstelensis Ak1 APBN01000001.1 | Coding AMP | BborAMP3 is a novel antimicrobial peptide gene found in the genome sequence of the thermophilic bacteria Brevibacillus borstelensis AK1. This AMP is known for its ability to inhibit Staphylococcus aureus. | Latifat Abdulsalam | 78bp | In testing |
| BBa_K5519005 | Brevibacillus borstelensis Ak1 APBN01000001.1 | Coding AMP | BborAMP4 is a novel antimicrobial peptide gene found in the genome sequence of the thermophilic bacteria Brevibacillus borstelensis AK1. This AMP is known for its ability to inhibit Staphylococcus aureus. | Latifat Abdulsalam | 138bp | In testing |
| BBa_K5519006 | Brevibacillus borstelensis Ak1 APBN01000001.1 | Coding AMP | BborAMP5 is a novel antimicrobial peptide gene found in the genome sequence of the thermophilic bacteria Brevibacillus borstelensis AK1. This AMP is known for its ability to inhibit Staphylococcus aureus. | Latifat Abdulsalam | 126bp | In testing |
| BBa_K5519007 | Brevibacillus borstelensis Ak1 APBN01000001.1 | Coding AMP | BborAMP6 is a novel antimicrobial peptide gene found in the genome sequence of the thermophilic bacteria Brevibacillus borstelensis AK1. This AMP is known for its ability to inhibit Staphylococcus aureus. | Latifat Abdulsalam | 69bp | In testing |