Let's start PLASTID PESTICIDES™ journey into PlantSynBio！

Hello

everyone!

PLASTID PESTICIDES™, developed by HUBU-China, is ready to share the journey of plastid-mediated RNAi (PM-RNAi) technology for pest control.

According to the Food and Agriculture Organization of the United Nations, pests destroy up to 40% of the world's crops, causing $220 billion in lose.

The extensive use of chemical pesticides not only creates serious resistance management problems, but also brings huge risks to environmental safety, food safety and human health.

Pest

More than 600 pests have been reported to be resistant to at least one pesticide, and their sensitivity to pesticides has decreased or even disappeared, resulting in reduced or ineffective pest control and farmers having to use more pesticides to protect crops

Gut

Long-term exposure to pesticides can be potentially harmful to human gut health, affect nervous system development, induce cancer, and cause damage to the reproductive system, liver, brain, and other parts of the body

Environment

When pesticides are sprayed, only about 30% fall on crops, and the remaining 70% spread to the soil and atmosphere. If the soil is polluted by pesticides for a long time, there will be obvious acidification, soil nutrient loss and soil porosity will become smaller, resulting in soil compaction, which will affect the growth of crops

Pests have developed biological resistance to existing control methods, and the use of RNAi technology to control insects is of great innovative significance.

NEW TECHNOLOGY

This year, HUBU-China also focuses on this issue, trying to manage Spodotera litura through PM-RNAi technology.

PM-RNAi

Description

In this project, we attempted to expressing artificial microRNA (amiRNA) targeting the chitin synthase 1 (CHS1) gene of S. litura from the genome of tobacco plastid. To protect the degradation of amiRNA caused by highly active nuclease in S. litura, amiRNA was delivered by a bacteriophage MS2 virus-like particle (VLP)-based delivery system.

RNAi technology is used to degrade target mRNA, resulting in gene silencing of S. litura, an important lepidopteran pest.

High

expression

Maternal

inheritance

No

positional

effect

Advantages

of

plastids

Nuclear

low

Copy number

plastid

Copy number

About

10000

No plastids in

pollen

MS2 Virus-like Particle (VLP)

The specific interaction between coat protein monomer of MS2 bacteriophage (CP) and pac site can package specific exogenous RNA.
The assembled MS2 VLP has a stable structure that can protect the target RNA from being degraded by nucleases.

PLASTID PESTICIDEST™

firmly believe that

plastid-mediated RNAi technology

is an effective approach

for pest insect management