A dual targeting nanobody made up of an EGFR and a HER2 nanobody linked by a GSSG linker.

A nanobody composed of two binding regions of EGFR and HER2 linked by a fragment of Fc region of the heavy chain of human IgG.

A nanobody of HERnb-Fc-EGFRnb carrying a secretory peptide for improved secretion.

A nanobody composed of two binding regions with a scrambled amino acid sequence that acts as a control for the dual targeting nanobody -Panobody.

The EGFR binding peptide is a 124 amino acid nanobody with a molecular weight of 13.4 kDa that specifically bind to EGFR.

The HER2 binding peptide is a 115 amino acid nanobody with a molecular weight of 12.6 kDa that specifically bind to HER2.

A scrambled peptide that acts as a control for the dual-targeting nanobody -Panobody.

A scrambled peptide that acts as a control for the dual-targeting nanobody -Panobody.

A secretory signalling peptide that facilitates the transport of Panobody to periplasm space of E. coli for secretion.

A fragment of Fc region in the heavy chain of human IgG connecting EGFR and HER2 nanobody.

A 3x GSSG linker connecting HER2 and EGFR nanobodies.

The T7 promoter is a DNA sequence derived from the T7 bacteriophage that is recognized by the T7 RNA polymerase enzyme for highly efficient transcription of DNA into RNA. Its function is to drive the expression of genes and production of recombinant proteins in molecular biology and biotechnology applications.

The Hexahistidine affinity tag is a short amino acid sequence that can be added to a recombinant protein to facilitate its purification using immobilized metal affinity chromatography (IMAC). It can bind to metal ions such as nickel or cobalt that are attached to a solid support, allowing for selective purification of the tagged protein from a complex mixture.

Ribosome binding site facilitates the binding of the ribosome to initiate translation of the mRNA into protein.

The T7 terminator is a short DNA sequence that signals the end of transcription by the T7 RNA polymerase enzyme. Its function is to ensure the accurate and efficient termination of transcription, preventing the synthesis of unwanted RNA transcripts downstream of the intended gene or sequence.