## Common Forward Primer for ribozyme connected parts: 5-tcagcaaattgtgaacatcatcacg-3

## Reverse Primers, and PCR with annealing temperature at 56 degree
R_nikA	5-GCCACGGGATCACCG-3
R_nikB	5-CCAGGTGCCGTACTGG-3
R_nikC	5-CGGGTCGCTGCCATC-3
R_nikD	5-GCGAAACCGGTTTGCC-3
R_nikE	5-CCGCGCCAGCTAATATTCC-3
R_hoxF	5-TGCACAAGTAAATCCGATACTTG-3
R_hoxH	5-GCACAGATGGTGACTGACG-3
R_hoxI	5-CGCTCATTGGTTTTTTCCCTG-3
R_hoxU	5-CCTCGACATTCAGGCCC-3
R_hoxW	5-TCTCTTGCGGCTGATCAG-3
R_hoxY	5-TGATATCGGTCAGCGACG-3
R_hypA	5-CAGCCGGCACCGG-3
R_hypB	5-GGGGCATGGGCACC-3
R_csoS2 5-TGGTAATCAGTGGGCACTTC-3
R_csoS3 5-ACGGTTCTTGACGCTATCC-3
R_csoS4A  5-CTCACCATTCCACTGATCAATAATCC-3
R_csoS4B  5-AGTTACCCAGTGATCGATGATG-3
R_csoS1A  5-ATCACCAACACGTTCGC-3
R_csoS1B  5-CATCGCCAACACGTTCAC-3
R_csoS1C  5-CCGACTCGTTCGCAGG-3
R_csoS1D  5-GCACGCTCGACTACCTG-3

## Forward Primer paired with R_cso: 5-atggggtcaaacatgcct-3

Our favourite parts are BBa_K5115067 and BBa_K5115068.

These two parts are outstanding examples of our expanding ribozyme-connected parts collection. Promoters and terminators are not included in these parts, and they were built in our plasmid backbones: BBa_K4765027 (T7 promoter with lac operator, T7 and rrnB T1 dual-terminator) and BBa_K5115069 (J23107, T7 and rrnB T1 dual-terminator). This flexible design enables all our ribozyme-connected parts easily transfer to other prokaryotic expression vectors, even shuttle vectors.

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Visit our Improve page for parts we improved this year.

Vist our Part Collection page for parts we made in 2024. Links to all parts available at gitlab and the Registry.

To check scencs behind our promotion video, please visit https://2024.igem.wiki/fudan/promotion-video/. Transcripts for our presentation video is on this Wiki.

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