iGEM Freiburg 2024
iGEM Freiburg 2024  | CAPTURE
  • Description Engineering Success Results Contribution
  • Protocols Notebook Safety
  • Part Collection Plasmid Design
  • Measurement
  • Human Practices
  • Team Members Attributions
  • Achievements

Notebook

Documented Experiments and Insights.
  • Cloning
  • Antimicrobial Peptides
  • Lipid-based Nanocarriers
  • Outer Membrane Vesicles

In this section, we welcome you to follow our lab journey, from the initial experiments to the final outcomes. Discover the highs and lows of our wet-lab work, carefully recorded to ensure scientific transparency. This year, we used eLabFTW as our digital notebook—an open-source electronic lab notebook designed for scientists to securely document, organize, and manage their research data. For comprehensive details and reproducibility, feel free to explore our publicly available lab notebook.

Cloning

pET-Plasmids

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

pUCP18-Plasmids

Link to PDF

Antimicrobial Peptide

Synthetic AMPs

Link to PDF

Link to PDF

Link to PDF

Expression of AMPs

Link to PDF

Link to PDF

HSTII-Characterization

Link to PDF

Growth curve

Link to PDF

Lipid-based Nanocarriers

Midori

Link to PDF

Link to PDF

Link to PDF

PicoGreen

Link to PDF

Plasmid Encapsulation

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Fusion

Link to PDF

Link to PDF

Link to PDF

Effect of Lipid-based Nanocarriers on Mammalian Cells

Link to PDF

Link to PDF

Link to PDF

Lipid-based Nanocarrier Production and Characterization

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Link to PDF

FACS

Link to PDF

Link to PDF

Outer Membrane Vesicles

Cloning

Link to PDF

Growth Curve

Link to PDF

OMV extraction

Link to PDF

Plasmid encapsulation

Link to PDF

Link to PDF

Link to PDF

Link to PDF

Protein Expression

Link to PDF

Fusion of OMVs with E. coli

Link to PDF

Effect OMVs on Mammalian Cells

Link to PDF

gitlab.igem.org/2024/freiburg | Creative Commons Attribution 4.0 License